O'Neal Lindsey, Schumann Peter, Falsen Enevold, Lawson Paul A
Department of Microbiology and Plant Biology, University of Oklahoma, 770 Van Vleet Oval, Norman, OK, 73019, USA.
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
Curr Microbiol. 2017 Apr;74(4):419-424. doi: 10.1007/s00284-016-1186-6. Epub 2017 Feb 13.
The entomopathogen "Corynebacterium okanaganae" was described by Lüthy in 1974 but the name was never validly published. Phylogenetic analysis employing 16S rRNA gene sequences demonstrate that "Corynebacterium okanaganae" is not a member of the genus Corynebacterium but related to members of the Microbacteriaceae being most closely related to, but distinct from, members of the genera Rathayibacter, Mycetocola and Curtobacterium. The bacterium is an aerobic, Gram-positive staining, rod-shaped actinobacterium with the cell-wall peptidoglycan based on 2,4, diaminobutyric acid as the diagnostic diamino acid. The predominant menaquinones are MK-10, MK-11 and MK-12, and the principle polar lipids are phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids consist of anteiso-C and anteiso-C. Therefore, based upon the phylogenetic, biochemical, and chemotaxonomic information, the organism merits recognition as a novel species and genus in the family Microbacteriaceae, for which the name Luethyella okanaganae gen. nov. sp. nov. is proposed. The type strain is LBG B4405 = CCUG 43304 = NCIMB 702272.
昆虫病原体“奥卡纳根棒状杆菌”由吕西于1974年描述，但该名称从未有效发表。采用16S rRNA基因序列的系统发育分析表明，“奥卡纳根棒状杆菌”不是棒状杆菌属的成员，而是与微杆菌科的成员相关，与拉氏杆菌属、分枝杆菌属和短小杆菌属的成员关系最为密切，但又有所不同。该细菌是一种需氧的、革兰氏阳性染色的杆状放线菌，其细胞壁肽聚糖以2,4-二氨基丁酸作为诊断性二氨基酸。主要的甲基萘醌是MK-10、MK-11和MK-12，主要的极性脂质是磷脂酰甘油和二磷脂酰甘油。主要脂肪酸由anteiso-C和anteiso-C组成。因此，基于系统发育、生化和化学分类学信息，该生物体应被确认为微杆菌科中的一个新物种和新属，为此提出了新属名Luethyella okanaganae gen. nov. sp. nov.。模式菌株是LBG B4405 = CCUG 43304 = NCIMB 702272。
