重组人白细胞介素-1β在体外改变视前区热敏神经元的活性。

Recombinant human interleukin-1 beta alters the activity of preoptic thermosensitive neurons in vitro.

作者信息

Nakashima T, Hori T, Mori T, Kuriyama K, Mizuno K

机构信息

Department of Physiology, Saga Medical School, Japan.

出版信息

Brain Res Bull. 1989 Sep;23(3):209-13. doi: 10.1016/0361-9230(89)90149-4.

DOI:10.1016/0361-9230(89)90149-4

PMID:2819478

Abstract

Effects of recombinant human interleukin-1 beta (rhIL-1 beta) on the single activity of thermosensitive and thermally insensitive neurons in the preoptic and anterior hypothalamus (PO/AH) were investigated in the rat's brain tissue slices. RhIL-1 beta (2.8 X 10(-9)-1.2 X 10(-8) M) decreased the activity in 19 of 27 warm-sensitive neurons and excited 2 of 3 cold-sensitive neurons, but had no effect on the majority (22 of 32) of thermally insensitive neurons. The neuronal responses to local rhIL-1 beta could be observed in a Ca2+ free/high Mg2+ solution, suggesting the postsynaptic actions of rhIL-1 beta. The actions of rhIL-1 beta on thermosensitive neurons were blocked or attenuated by concurrent application of sodium salicylate, but not by naloxone. The results suggest that centrally formed IL-1 beta may induce fever by its actions on PO/AH thermosensitive neurons, which do not involve the opioid receptor mechanisms.

摘要

在大鼠脑组织切片中研究了重组人白细胞介素-1β（rhIL-1β）对视前区和下丘脑前部（PO/AH）热敏和热不敏感神经元单一活动的影响。rhIL-1β（2.8×10⁻⁹ - 1.2×10⁻⁸ M）使27个温敏神经元中的19个活动降低，使3个冷敏神经元中的2个兴奋，但对大多数（32个中的22个）热不敏感神经元没有影响。在无钙/高镁溶液中可观察到神经元对局部rhIL-1β的反应，提示rhIL-1β的突触后作用。rhIL-1β对热敏神经元的作用可被同时应用水杨酸钠阻断或减弱，但不能被纳洛酮阻断。结果表明，中枢产生的IL-1β可能通过其对视前区/下丘脑前部热敏神经元的作用诱导发热，这一过程不涉及阿片受体机制。