Biotic and Abiotic Stresses Activate Different Ca Permeable Channels in .

To survive, plants must respond rapidly and effectively to various stress factors, including biotic and abiotic stresses. Salinity stress triggers the increase of cytosolic free Ca concentration ([Ca]) via Ca influx across the plasma membrane, as well as bacterial flg22 and plant endogenous peptide Pep1. However, the interaction between abiotic stress-induced [Ca] increases and biotic stress-induced [Ca] increases is still not clear. Employing an aequorin-based Ca imaging assay, in this work, we investigated the [Ca] changes in response to flg22, Pep1, and NaCl treatments in . We observed an additive effect on the [Ca] increase which induced by flg22, Pep1, and NaCl. Our results indicate that biotic and abiotic stresses may activate different Ca permeable channels. Further, calcium signal induced by biotic and abiotic stresses was independent in terms of spatial and temporal patterning.