Hussein Waleed M, Mukaida Saori, Azmi Fazren, Bartlett Stacey, Olivier Celine, Batzloff Michael R, Good Michael F, Skwarczynski Mariusz, Toth Istvan
School of Chemistry and Molecular Biosciences, The University of Queensland , Brisbane, QLD 4072, Australia.
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia; Faculty of Pharmacy, National University of Malaysia, Kuala Lumpur, Malaysia.
ACS Med Chem Lett. 2017 Jan 24;8(2):227-232. doi: 10.1021/acsmedchemlett.6b00453. eCollection 2017 Feb 9.
Safe immunostimulants (adjuvants) are essential for the development of highly potent peptide-based vaccines. This study examined for the first time whether fluorinated lipids could stimulate humoral immunity in vivo when conjugated to peptide antigen. The impact of fluorination on humoral immunity was tested using a library of peptide-based vaccine candidates against the group A streptococcus (GAS). The fluorinated constructs stimulated similar mouse IgG titers to those elicited by complete Freund's adjuvant (CFA) and were higher than those produced in mice that received the nonfluorinated constructs.
安全的免疫刺激剂（佐剂）对于高效肽基疫苗的研发至关重要。本研究首次检测了氟化脂质与肽抗原偶联后能否在体内刺激体液免疫。使用一组针对A群链球菌（GAS）的肽基候选疫苗，测试了氟化对体液免疫的影响。氟化构建体刺激产生的小鼠IgG滴度与完全弗氏佐剂（CFA）诱导的相似，且高于接受非氟化构建体的小鼠所产生的滴度。
