Department of Chemical Engineering and Biotechnology, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.
Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Chemistry. 2017 Apr 11;23(21):4990-4994. doi: 10.1002/chem.201700673. Epub 2017 Mar 27.
Oligonucleotides containing photo-caged dienes were prepared and shown to react quantitatively in a light-induced Diels-Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub-micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written by direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilization. This mild and efficient light-driven platform technology holds promise for the fabrication of complex bioarrays with sub-micron resolution.
合成了含有光致笼型二烯的寡核苷酸，并证明其在水溶液中可在数分钟内与功能马来酰亚胺定量发生光诱导 Diels-Alder 环加成反应。由于其高收率和快速反应速率，该反应被用于通过直接激光写入 (DLW) 对 DNA 表面进行亚微米分辨率的图案化。通过直接激光写入 (DLW) 以可变模式写入功能 DNA 阵列，然后通过 DNA 定向固定化进一步用荧光团和蛋白质进行编码。这种温和高效的光驱动平台技术有望用于制造具有亚微米分辨率的复杂生物阵列。
