Department of Gastroenterology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, Guangdong 510080, China.
Department of Gastroenterology, The 101 Hospital of the Chinese People\'s Liberation Army, Wuxi, Jiangsu 214000, China.
Front Biosci (Landmark Ed). 2017 Mar 1;22(8):1365-1378. doi: 10.2741/4549.
The purpose of this study was to assess the anti-tumor effects of macrophage migration inhibitory factor (MIF) siRNA on colorectal cancer in a mouse xenograft model. MIF specific siRNA (MIF siRNA) or a nonspecific control siRNA was introduced to murine colorectal cancer CT-26 cells. Mouse xenograft models of colorectal cancer were established. MIF siRNA, control siRNA or water was injected twice a week intravenously for 4 weeks. MIF siRNA inhibited the proliferation and migration, while induced apoptosis of CT-26 cells . Injection of MIF siRNA resulted in a significant decrease of serum MIF and VEGF levels, and the weight and volume of cecum-grafted tumors . In contrast, the number of apoptotic cells and caspase-3 expression were increased by MIF siRNA in cecum graft tumor tissues. Moreover, the water and fodder consumption were significantly improved by MIF siRNA treatment. Importantly, MIF siRNA reduced the hepatic metastases from colorectal cancer. Our results suggest that siRNA targeting MIF is a promising agent for the treatment of hepatic metastasis of colorectal cancer cells.
本研究旨在评估巨噬细胞移动抑制因子（MIF）siRNA 在小鼠异种移植模型中对结直肠癌的抗肿瘤作用。将 MIF 特异性 siRNA（MIF siRNA）或非特异性对照 siRNA 导入鼠结直肠癌细胞 CT-26 中。建立结直肠癌的小鼠异种移植模型。每周两次静脉内注射 MIF siRNA、对照 siRNA 或水 4 周。MIF siRNA 抑制 CT-26 细胞的增殖和迁移，同时诱导细胞凋亡。注射 MIF siRNA 导致血清 MIF 和 VEGF 水平显著降低，盲肠移植瘤的重量和体积也降低。相比之下，MIF siRNA 增加了盲肠移植瘤组织中的细胞凋亡和 caspase-3 表达。此外，MIF siRNA 治疗还显著改善了水和饲料的消耗。重要的是，MIF siRNA 减少了结直肠癌的肝转移。我们的结果表明，靶向 MIF 的 siRNA 是治疗结直肠癌细胞肝转移的一种有前途的药物。
