肿瘤坏死因子-α（-308G>A）和肿瘤坏死因子受体1（36A>G）单核苷酸多态性是牙源性角化囊性瘤发生发展的强烈危险因素。

TNF-α (-308G>A) and TNF-R1 (36A>G) single nucleotide polymorphisms are strong risk factors for odontogenic keratocystic tumor development.

作者信息

Ilic Branislav, Nikolic Nadja, Andric Miroslav, Jelovac Drago, Milicic Biljana, Jozic Tanja, Krstic Slobodan, Milasin Jelena

机构信息

Clinic for Oral Surgery, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.

Department of Human Genetics, School of Dental Medicine, University of Belgrade, Belgrade, Serbia.

出版信息

J Oral Pathol Med. 2017 Apr;46(4):292-296. doi: 10.1111/jop.12564. Epub 2017 Mar 5.

DOI:10.1111/jop.12564

PMID:28199753

Abstract

BACKGROUND

Polymorphisms in genes encoding tumor necrosis factor-α (TNF-α) and its receptor TNF-R1 have been shown to affect one person's susceptibility to develop certain neoplastic diseases. The aim of the present association study was to investigate whether single nucleotide polymorphisms (SNPs) in TNF-α (-308G>A) and TNF-R1 (36A>G) genes modulate the susceptibility for keratocystic odontogenic tumors (KCOTs) development in Serbian patients.

METHODS

Genotyping was performed in 60 KCOT patients and 125 healthy individuals, using polymerase chain reaction/restriction fragment length polymorphism analysis.

RESULTS

A significant difference in genotype and allele frequencies was found between patients and controls for both SNPs (P < 0.05). Carriers of the TNF-α A variant had an eightfold increase of KCOT risk (OR = 8.12, 95% CI = 3.98-16.56, P < 0.0001), while carriers of the TNF-R1 G variant had approximately a fourfold increase of KCOT risk (OR=3.65, CI: 1.60-8.40, P = 0.001).

CONCLUSIONS

Our findings suggest that the two polymorphisms are strong risk factors for KCOT development in Serbian population.

摘要

背景

编码肿瘤坏死因子-α（TNF-α）及其受体TNF-R1的基因多态性已被证明会影响一个人患某些肿瘤性疾病的易感性。本关联研究的目的是调查TNF-α（-308G>A）和TNF-R1（36A>G）基因中的单核苷酸多态性（SNP）是否会调节塞尔维亚患者发生牙源性角化囊性瘤（KCOT）的易感性。

方法

采用聚合酶链反应/限制性片段长度多态性分析对60例KCOT患者和125例健康个体进行基因分型。

结果

两个SNP的患者和对照组之间在基因型和等位基因频率上均存在显著差异（P<0.05）。TNF-α A变异体携带者患KCOT的风险增加了八倍（OR=8.12，95%CI=3.98-16.56，P<0.0001），而TNF-R1 G变异体携带者患KCOT的风险增加了约四倍（OR=3.65，CI：1.60-8.40，P=0.001）。