Testa I, Rabini R A, Corvetta A, Danieli G
Scand J Rheumatol. 1987;16(4):301-5. doi: 10.3109/03009748709102932.
The activity of the plasma membrane Na+/K+-ATPase and cellular sodium (Nai) and potassium (Ki) content were analysed in RBCs from 15 rheumatoid arthritis (RA) and 30 reference subjects (11 healthy controls, 12 osteoarthritis and 7 gouty patients). Na+/K+-ATPase activity was determined by measuring the inorganic phosphate (Pi) released by incubation in a reaction medium in the presence and absence of K ions or ouabain. Nai and Ki were measured with an ion-selective electrode analyser on the hemolysates, after washing the RBCs in 110 mM MgCl2. The Na/K-ATPase activity was significantly lower in RA patients than in both healthy controls and patients with osteoarthritis or gout. A slight but significant increase in Nai was observed in rheumatoid subjects. It is hypothesized that the decrease in the Na+/K+-ATPase activity in RA may be the result of a defective expression of membrane proteins, which is probably related to the altered cell sensitivity observed.
对15例类风湿性关节炎（RA）患者及30例对照受试者（11例健康对照者、12例骨关节炎患者和7例痛风患者）红细胞中的质膜钠/钾-ATP酶活性、细胞内钠（Nai）和钾（Ki）含量进行了分析。通过测量在有钾离子或哇巴因存在及不存在的情况下，在反应介质中孵育释放的无机磷酸盐（Pi）来测定钠/钾-ATP酶活性。在110 mM氯化镁中洗涤红细胞后，用离子选择性电极分析仪测量溶血产物中的Nai和Ki。RA患者的钠/钾-ATP酶活性显著低于健康对照者以及骨关节炎或痛风患者。类风湿受试者的Nai略有但显著增加。据推测，RA中钠/钾-ATP酶活性降低可能是膜蛋白表达缺陷的结果，这可能与观察到的细胞敏感性改变有关。
