Pilcher G D, Gutshall D M, Langley A E
Toxicol Appl Pharmacol. 1987 Sep 15;90(2):198-205. doi: 10.1016/0041-008x(87)90327-9.
Perfluoro-n-decanoic acid (PFDA) is a member of a family of surfactants with numerous industrial applications. The acute toxicity of PFDA is characterized by body wasting and delayed lethality. Recent reports have indicated that the effects of PFDA may involve an action on the structure of biological membranes which results in an alteration of function. In the present study we extend our work on the membrane actions of PFDA by examining its effects on myocardial beta-adrenoceptor binding characteristics and adenylate cyclase. Following a single injection of PFDA the apparent number of beta-receptor binding sites was reduced compared to pair-fed controls. This change in beta-receptor binding capacity was reflected in a reduced ability of norepinephrine to activate adenylate cyclase. No alterations were observed in basal adenylate cyclase activity or in the ability of NaF or guanylyl imidodiphosphate to stimulate the enzyme. The fatty acid composition of the heart was changed by PFDA treatment. Our results suggest that the toxic effects of PFDA may be due to an alteration of the membrane lipid bilayer leading to changes in the functional activity of myocardial membranes.
全氟正癸酸（PFDA）是一类具有众多工业应用的表面活性剂家族的成员。PFDA的急性毒性表现为体重减轻和延迟致死。最近的报告表明，PFDA的作用可能涉及对生物膜结构的影响，从而导致功能改变。在本研究中，我们通过研究PFDA对心肌β-肾上腺素能受体结合特性和腺苷酸环化酶的影响，扩展了我们对PFDA膜作用的研究。单次注射PFDA后，与配对喂食的对照组相比，β-受体结合位点的表观数量减少。β-受体结合能力的这种变化反映在去甲肾上腺素激活腺苷酸环化酶的能力降低。未观察到基础腺苷酸环化酶活性或氟化钠或鸟苷酰亚胺二磷酸刺激该酶的能力发生改变。PFDA处理改变了心脏的脂肪酸组成。我们的结果表明，PFDA的毒性作用可能是由于膜脂质双层的改变导致心肌膜功能活性的变化。
