Nesbakken T, Kapperud G, Sørum H, Dommarsnes K
Acta Pathol Microbiol Immunol Scand B. 1987 Jun;95(3):167-73. doi: 10.1111/j.1699-0463.1987.tb03107.x.
Restriction endonuclease analysis (BamHI and EcoRI) was used to investigate the structural variability of 40-50 Mdal plasmids from 129 Yersinia enterocolitica strains isolated from human patients, animals, and food in 12 countries in Europe, North America, and Asia. A total of 12 restriction patterns was detected among plasmids from the six serogroups examined. The DNA fragment profiles were found to vary, not only between serogroups, but also among plasmids isolated from strains with the same serogroup affiliation. Despite widespread geographical and ecological origin, the plasmids isolated from the 0:3 and 0:9 strains examined revealed a surprising stability, whereas plasmids from 0:8 and 0:5,27 showed substantial diversity. The genes associated with autoagglutination, calcium dependency, and mouse virulence were conserved in all 12 plasmid variants detected. Our finding that porcine and human isolates harboured plasmids with identical restriction patterns, provides additional support for the importance of pigs in the epidemiology of human Y. enterocolitica infection.
采用限制性内切酶分析（BamHI和EcoRI）研究了从欧洲、北美和亚洲12个国家的人类患者、动物和食物中分离出的129株小肠结肠炎耶尔森氏菌中40 - 50 Mdal质粒的结构变异性。在所检测的六个血清群的质粒中总共检测到12种限制性酶切图谱。发现DNA片段谱不仅在血清群之间不同，而且在从具有相同血清群归属的菌株中分离出的质粒之间也不同。尽管地理和生态来源广泛，但从所检测的0:3和0:9菌株中分离出的质粒显示出惊人的稳定性，而来自0:8和0:5,27的质粒则表现出很大的多样性。与自凝、钙依赖性和小鼠毒力相关的基因在检测到的所有12种质粒变体中都是保守的。我们发现猪和人类分离株携带具有相同限制性酶切图谱的质粒，这为猪在人类小肠结肠炎耶尔森氏菌感染流行病学中的重要性提供了额外的支持。
