Odinot P T, Meis J F, Van den Hurk P J, Hoogkamp-Korstanje J A, Melchers W J
University Hospital Nijmegen, Department of Medical Microbiology, The Netherlands.
Epidemiol Infect. 1995 Oct;115(2):269-77. doi: 10.1017/s0950268800058398.
PCR-based DNA fingerprinting was used to characterize 48 clinical isolates of Yersinia enterocolitica. The samples were examined by random amplified polymorphic DNA (RAPD-PCR) and inter-repeat PCR (IR-PCR). IR-PCR with two enterobacterial repetitive intergenic consensus primers resulted in patterns which were poorly discriminated; 2 of 11 arbitrary primers (RAPD-PCR) provided sufficient discriminatory power. In comparisons with serotyping and biotyping, RAPD-fingerprinting was the most discriminatory technique and may therefore be a valuable epidemiological tool for the study of Y. enterocolitica infections.
基于聚合酶链反应（PCR）的DNA指纹图谱技术被用于鉴定48株小肠结肠炎耶尔森菌临床分离株。通过随机扩增多态性DNA（RAPD-PCR）和重复序列间PCR（IR-PCR）对样本进行检测。使用两种肠道细菌重复基因间共有引物进行的IR-PCR所产生的图谱鉴别能力较差；11种随机引物中有2种（RAPD-PCR）具有足够的鉴别能力。与血清分型和生物分型相比，RAPD指纹图谱技术的鉴别能力最强，因此可能是研究小肠结肠炎耶尔森菌感染的一种有价值的流行病学工具。
