在兔关节软骨缺损模型中使用热可逆凝胶化聚合物扩增的自体软骨细胞移植
Transplantation of autologous chondrocytes expanded using Thermoreversible Gelation Polymer in a rabbit model of articular cartilage defect.
作者信息
Arumugam Sivaraman, Bhupesh Karthik Balasubramanyan, Chinnuswami Rajeswar, Mori Yuichi, Yoshioka Hiroshi, Senthilkumar Rajappa, Mathaiyan Rajmohan, Ramalingam Karthick, Senthilkumar Preethy, Abraham Samuel J K
机构信息
Sri Ramachandra Arthroscopy & Sports Sciences Centre (SRASSC), Sri Ramachandra University, Chennai, India.
Mebiol Inc., Hiratsuka, Japan.
出版信息
J Orthop. 2017 Feb 3;14(2):223-225. doi: 10.1016/j.jor.2017.01.003. eCollection 2017 Jun.
Graft failure due to de-differentiation of the chondrocytes during in vitro culture and after transplantation is a major hurdle in Autologous Chondrocyte Implantation (ACI). We, herein, report the transplantation of autologous chondrocytes expanded using a Thermo-reversible Gelation Polymer (TGP) in a rabbit model. A full thickness chondral defect was created in one of the knee joints in each of the six rabbits of the study and autologous chondrocytes expanded using TGP scaffold were transplanted after 10 weeks. H & E staining of the biopsy after 6 months revealed maintenance of articular cartilage phenotype.
在体外培养及移植后，软骨细胞去分化导致的移植物失败是自体软骨细胞移植（ACI）的一个主要障碍。在此，我们报告了在兔模型中使用热可逆凝胶聚合物（TGP）扩增的自体软骨细胞的移植情况。在该研究的六只兔子中，每只兔子的一个膝关节制造了一个全层软骨缺损，并在10周后移植了使用TGP支架扩增的自体软骨细胞。6个月后活检的苏木精-伊红染色显示关节软骨表型得以维持。
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