一种新型聚合物支架的三维体外培养环境，可从受骨关节炎影响的软骨组织衍生的人软骨细胞中产生软骨祖细胞和间充质干细胞。
A three-dimensional in vitro culture environment of a novel polymer scaffold, yielding chondroprogenitors and mesenchymal stem cells in human chondrocytes derived from osteoarthritis-affected cartilage tissue.
作者信息
Katoh Shojiro, Yoshioka Hiroshi, Iwasaki Masaru, Senthilkumar Rajappa, Rajmohan Mathaiyan, Karthick Ramalingam, Preethy Senthilkumar, Abraham Samuel Jk
机构信息
Edogawa Evolutionary Lab of Science, Edogawa Hospital Campus, 2-24-18, Higashi Koiwa, Edogawa-Ku, Tokyo, 133-0052, Japan.
Department of Orthopaedic Surgery, Edogawa Hospital, 2-24-18, Higashi Koiwa, Edogawa-Ku, Tokyo, 133-0052, Japan.
出版信息
J Orthop. 2021 Jan 16;23:138-141. doi: 10.1016/j.jor.2021.01.005. eCollection 2021 Jan-Feb.
Abstract
OBJECTIVE
We evaluated the expression of stem/progenitor biomarkers in osteoarthritic tissue derived chondrocytes cultured using a three-dimensional (3D) thermo-reversible gelation polymer (TGP).
METHODS
The chondrocytes from discarded biopsy tissues obtained from human elderly patients with osteoarthritis were cultured using the 3D-TGP up to six weeks.
RESULTS
The chondrocytes grew in a tissue-like manner, without de-differentiation into fibroblasts, and the cells thus tissue-engineered were proven positive for CD49e, OCT4, CD-105 and STRO-1 by immunohistochemistry.
CONCLUSION
This study establishes the efficacy of this 3D-TGP platform for clinically useable tissue-engineered cartilage for improvising the clinical outcome of cell therapy for cartilage repair.
摘要
目的
我们评估了使用三维（3D）热可逆凝胶化聚合物（TGP）培养的骨关节炎组织来源软骨细胞中干/祖细胞生物标志物的表达。
方法
使用3D-TGP培养从老年骨关节炎患者废弃活检组织中获取的软骨细胞，培养时间长达六周。
结果
软骨细胞以组织样方式生长，未分化为成纤维细胞，通过免疫组织化学证明，如此构建的组织工程化细胞对CD49e、OCT4、CD-105和STRO-1呈阳性。
结论
本研究确立了该3D-TGP平台用于临床可用的组织工程化软骨以改善软骨修复细胞治疗临床效果的有效性。
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Acta Histochem. 2020 Jan;122(1):151462. doi: 10.1016/j.acthis.2019.151462. Epub 2019 Nov 13.
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