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作为诊断生物标志物的miR-17调节乳腺癌细胞的增殖。

miR-17 as a diagnostic biomarker regulates cell proliferation in breast cancer.

作者信息

Yang Fangliang, Li Yuan, Xu Lingyun, Zhu Yulan, Gao Haiyan, Zhen Lin, Fang Lin

机构信息

Department of Thyroid and Breast Surgery, Changzhou No 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou; Department of Thyroid and Breast Surgery, Shanghai No 10 People's Hospital, Clinical College of Nanjing Medical University, Shanghai, People's Republic of China.

Department of Thyroid and Breast Surgery, Changzhou No 2 People's Hospital Affiliated to Nanjing Medical University, Changzhou.

出版信息

Onco Targets Ther. 2017 Jan 27;10:543-550. doi: 10.2147/OTT.S127723. eCollection 2017.

DOI:10.2147/OTT.S127723
PMID:28203087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5293507/
Abstract

BACKGROUND

MicroRNAs (miRNAs) have been shown to be involved in the initiation and progression of cancers in the literature. In this study, we aimed to evaluate the clinicopathological role of miR-17 in breast cancer.

MATERIALS AND METHODS

The expression of miR-17 was measured in 132 breast cancer tissues and paired adjacent normal tissues by using real-time quantitative polymerase chain reaction. The association between miR-17 expression levels and clinicopathological parameters was also analyzed. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays were used to investigate the role of miR-17 in the regulation of breast cancer cells.

RESULTS

The expression of miR-17 was remarkably increased in breast cancer tissues and cell lines. Clinical association analysis revealed that a high expression of miR-17 was prominently associated with poor survival time in breast cancer. Overexpression of miR-17 promoted cell proliferation and induced tumor growth.

CONCLUSION

Our findings clarified that the upregulation of miR-17 played a vital role in breast cancer progression and suggested that miR-17 could be used as a prognostic biomarker for breast cancer.

摘要

背景

文献表明，微小RNA（miRNA）参与癌症的发生和发展。在本研究中，我们旨在评估miR-17在乳腺癌中的临床病理作用。

材料与方法

采用实时定量聚合酶链反应检测132例乳腺癌组织及其配对的癌旁正常组织中miR-17的表达。分析miR-17表达水平与临床病理参数之间的相关性。采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法和流式细胞术检测miR-17在乳腺癌细胞调控中的作用。

结果

miR-17在乳腺癌组织和细胞系中表达显著增加。临床相关性分析显示，miR-17高表达与乳腺癌患者较差的生存时间显著相关。miR-17过表达促进细胞增殖并诱导肿瘤生长。

结论

我们的研究结果表明，miR-17的上调在乳腺癌进展中起重要作用，提示miR-17可作为乳腺癌的预后生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/cef31788dd2c/ott-10-543Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/439e7cc6c8ff/ott-10-543Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/39343c9a1bfe/ott-10-543Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/2b15a57e05aa/ott-10-543Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/cef31788dd2c/ott-10-543Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/439e7cc6c8ff/ott-10-543Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/39343c9a1bfe/ott-10-543Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/2b15a57e05aa/ott-10-543Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d890/5293507/cef31788dd2c/ott-10-543Fig4.jpg

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