环磷酸腺苷诱导小鼠乳酸脱氢酶 -A 启动子 - 氯霉素乙酰转移酶融合基因在中国仓鼠卵巢野生型细胞中表达，但在环磷酸腺苷依赖性蛋白激酶突变细胞中不表达。
Cyclic AMP-induced expression of the mouse lactate dehydrogenase-A promoter-cat fusion gene in Chinese hamster ovary wild-type cells, but not in cAMP-dependent protein kinase mutant cells.
作者信息
Hou E W, Li S S
出版信息
Biochem Biophys Res Commun. 1987 Aug 31;147(1):501-5. doi: 10.1016/s0006-291x(87)80149-3.
PMID:2820403
Abstract
The promoter region of mouse lactate dehydrogenase-A gene was fused with the chloramphenicol acetyltransferase gene of Escherichia coli, and the expression of this fusion gene was induced by cyclic AMP in Chinese hamster ovary wild-type cells, but not in mutants affecting the regulatory or catalytic subunit of cAMP-dependent protein kinase.
摘要
将小鼠乳酸脱氢酶 -A 基因的启动子区域与大肠杆菌的氯霉素乙酰转移酶基因融合，此融合基因的表达在中华仓鼠卵巢野生型细胞中可被环磷酸腺苷（cAMP）诱导，但在影响 cAMP 依赖性蛋白激酶调节或催化亚基的突变体中则不能被诱导。
Zotero 插件