Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
Biotechnol Adv. 2013 Dec;31(8):1501-13. doi: 10.1016/j.biotechadv.2013.07.007. Epub 2013 Aug 2.
microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines.
微小 RNA 能够调节控制细胞行为和表型的复杂途径，它们被提议作为生物制药生产细胞系（特别是中国仓鼠卵巢细胞）优化中细胞工程的潜在靶点。然而，直到最近，由于缺乏关于这条工业上重要的细胞系的基因组序列信息，研究受到限制。自 2011 年以来，随着 CHO 细胞基因组序列和其他相关数据集的发表，人们对 CHO 细胞生理学有了更深入的了解，并开发出了用于新型工程策略的必要工具。在本综述中，我们讨论了从其他生物模型获得的微小 RNA 调控机制的知识，以及已经在 CHO 细胞上进行的概念验证，从而为微小 RNA 工程在生产细胞系中的潜在应用提供了展望。
