Chang W C, Su G W
Biochem Pharmacol. 1987 Sep 15;36(18):3033-6. doi: 10.1016/0006-2952(87)90220-6.
In the present study, the lipoxygenase activity in polymorphonuclear (PMN) leukocytes of rats was characterized, and the change in lipoxygenase activity in peritoneal and peripheral PMN leukocytes challenged by caseinate was investigated. Peritoneal PMN leukocytes produced 5-HETE and LTB4 as the major 5-lipoxygenase metabolites from arachidonic acid. The 5-lipoxygenase activity was calcium dependent. Caseinate treatment in rats significantly stimulated the 5-lipoxygenase activity in peripheral and peritoneal PMN leukocytes. Although it also stimulated the cyclooxygenase metabolic pathway, the effect was not as evident as that on the 5-lipoxygenase pathway. Since LTB4 is a potent chemotactic factor for PMN leukocyte migration, the present results might explain the mechanism on the PMN leukocyte infiltration caused by caseinate treatment.
在本研究中，对大鼠多形核（PMN）白细胞中的脂氧合酶活性进行了表征，并研究了酪蛋白酸盐刺激后腹膜和外周PMN白细胞中脂氧合酶活性的变化。腹膜PMN白细胞从花生四烯酸产生5-羟基二十碳四烯酸（5-HETE）和白三烯B4（LTB4）作为主要的5-脂氧合酶代谢产物。5-脂氧合酶活性依赖于钙。大鼠经酪蛋白酸盐处理后，显著刺激了外周和腹膜PMN白细胞中的5-脂氧合酶活性。虽然它也刺激了环氧化酶代谢途径，但其作用不如对5-脂氧合酶途径明显。由于LTB4是PMN白细胞迁移的有效趋化因子，本研究结果可能解释了酪蛋白酸盐处理导致PMN白细胞浸润的机制。
