• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Jesridonin对紫杉醇耐药的EC109人食管癌细胞的抗增殖作用。

Anti-proliferative effect of Jesridonin on paclitaxel-resistant EC109 human esophageal carcinoma cells.

作者信息

Wang Cong, Guo Liubin, Wang Saiqi, Wang Junwei, Li Yongmei, Dou Yinhui, Wang Ran, Shi Hongge, Ke Yu, Liu Hongmin

机构信息

Key Laboratory of Technology of Drug Preparation (Zhengzhou University), Ministry of Education of China, Zhengzhou, Henan 450001, P.R. China.

Department of Basic Medicine, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, P.R. China.

出版信息

Int J Mol Med. 2017 Mar;39(3):645-653. doi: 10.3892/ijmm.2017.2867. Epub 2017 Jan 24.

DOI:10.3892/ijmm.2017.2867
PMID:28204832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5360389/
Abstract

Chemoresistance to anticancer drugs is a major obstacle in the efforts to develop a successful treatment strategy for esophageal squamous carcinoma (ESCC). Thus, the exploration of new drugs and treatment strategies for combating resistance are of utmost importance. In this study, we investigated the antitumor drug resistance activity of Jesridonin, a new ent-kaurene diterpenoid, and its possible mechanisms of action using the resistant cancer cell line, EC109/Taxol. MTT assay revealed that Jesridonin had similar IC50 values against EC109 paclitaxel-sensitive cells and drug-resistant EC109/Taxol cells in vitro. In mice, Jesridonin effectively prevented the growth of EC109/Taxol tumor xenografts without exerting any significant toxicity. In addition, Jesridonin significantly inhibited the proliferation of EC109/Taxol cells, induced apoptosis and arrested the cell cycle at the G2/M phase. Furthermore, western blot analysis revealed that Jesridonin upregulated the expression of p53, p53 upregulated modulator of apoptosis (PUMA), cleaved-caspase-9 and cleaved-caspase-3 in EC109/Taxol cells, and downregulated the expression of procaspase-3, procaspase-9 and Bcl-2 in the EC109/Taxol cells in a concentration-dependent manner. Overall, our results demonstrate that Jesridonin may have potential for use in the treatment of paclitaxel-resistant ESCC. The data of the present study may lead to the development of novel treatment strategies for paclitaxel-resistant tumors.

摘要

对抗癌药物的化学抗性是为食管鳞状细胞癌（ESCC）制定成功治疗策略的主要障碍。因此，探索对抗抗性的新药和治疗策略至关重要。在本研究中，我们使用耐药癌细胞系EC109/Taxol研究了一种新的对映-贝壳杉烯二萜类化合物Jesridonin的抗肿瘤耐药活性及其可能的作用机制。MTT分析显示，Jesridonin在体外对EC109紫杉醇敏感细胞和耐药的EC109/Taxol细胞具有相似的IC50值。在小鼠中，Jesridonin有效地抑制了EC109/Taxol肿瘤异种移植瘤的生长，且未产生任何明显毒性。此外，Jesridonin显著抑制EC109/Taxol细胞的增殖，诱导细胞凋亡并使细胞周期停滞在G2/M期。此外，蛋白质印迹分析显示，Jesridonin上调了EC109/Taxol细胞中p53、p53上调的凋亡调节因子（PUMA）、裂解的半胱天冬酶-9和裂解的半胱天冬酶-3的表达，并以浓度依赖性方式下调了EC109/Taxol细胞中前半胱天冬酶-3、前半胱天冬酶-9和Bcl-2的表达。总体而言，我们的结果表明Jesridonin可能具有用于治疗紫杉醇耐药性ESCC的潜力。本研究的数据可能会导致开发针对紫杉醇耐药性肿瘤的新治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/1fa16b8b5baa/IJMM-39-03-0645-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/8839247ab83c/IJMM-39-03-0645-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/28db2494e18b/IJMM-39-03-0645-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/51efe81132ab/IJMM-39-03-0645-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/f8609302d899/IJMM-39-03-0645-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/9da0bfa54530/IJMM-39-03-0645-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/1fa16b8b5baa/IJMM-39-03-0645-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/8839247ab83c/IJMM-39-03-0645-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/28db2494e18b/IJMM-39-03-0645-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/51efe81132ab/IJMM-39-03-0645-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/f8609302d899/IJMM-39-03-0645-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/9da0bfa54530/IJMM-39-03-0645-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdd2/5360389/1fa16b8b5baa/IJMM-39-03-0645-g05.jpg

相似文献

1
Anti-proliferative effect of Jesridonin on paclitaxel-resistant EC109 human esophageal carcinoma cells.Jesridonin对紫杉醇耐药的EC109人食管癌细胞的抗增殖作用。
Int J Mol Med. 2017 Mar;39(3):645-653. doi: 10.3892/ijmm.2017.2867. Epub 2017 Jan 24.
2
Establishment and characterization of a paclitaxel‑resistant human esophageal carcinoma cell line.紫杉醇耐药人食管癌细胞系的建立与鉴定。
Int J Oncol. 2013 Nov;43(5):1607-17. doi: 10.3892/ijo.2013.2083. Epub 2013 Aug 29.
3
Jesridonin in combination with paclitaxel demonstrates synergistic anti-tumor activity in human esophageal carcinoma cells.耶斯瑞多宁与紫杉醇联合使用在人食管癌细胞中显示出协同抗肿瘤活性。
Bioorg Med Chem Lett. 2017 May 1;27(9):2058-2062. doi: 10.1016/j.bmcl.2017.02.008. Epub 2017 Feb 7.
4
The Antitumor Activity of the Novel Compound Jesridonin on Human Esophageal Carcinoma Cells.新型化合物杰斯里多宁对人食管癌细胞的抗肿瘤活性
PLoS One. 2015 Jun 23;10(6):e0130284. doi: 10.1371/journal.pone.0130284. eCollection 2015.
5
Bardoxolone methyl induces apoptosis and autophagy and inhibits epithelial-to-mesenchymal transition and stemness in esophageal squamous cancer cells.巴多昔芬甲酯可诱导食管鳞状癌细胞凋亡和自噬，并抑制其上皮-间质转化和干性。
Drug Des Devel Ther. 2015 Feb 17;9:993-1026. doi: 10.2147/DDDT.S73493. eCollection 2015.
6
OP16, a novel ent-kaurene diterpenoid, potentiates the antitumor effect of rapamycin by inhibiting rapamycin-induced feedback activation of Akt signaling in esophageal squamous cell carcinoma.OP16，一种新型的 ent-贝壳杉烯二萜，通过抑制雷帕霉素诱导的食管鳞癌细胞中 Akt 信号的反馈激活，增强雷帕霉素的抗肿瘤作用。
Biochem Pharmacol. 2017 Sep 15;140:16-27. doi: 10.1016/j.bcp.2017.05.013. Epub 2017 May 21.
7
Establishment and biological analysis of the EC109/CDDP multidrug-resistant esophageal squamous cell carcinoma cell line.EC109/顺铂多药耐药食管鳞状细胞癌细胞系的建立及生物学分析
Oncol Rep. 2009 Jul;22(1):65-71. doi: 10.3892/or_00000407.
8
Xerophilusin B induces cell cycle arrest and apoptosis in esophageal squamous cell carcinoma cells and does not cause toxicity in nude mice.嗜旱菌素B诱导食管鳞状细胞癌细胞的细胞周期停滞和凋亡，且对裸鼠无毒性。
J Nat Prod. 2015 Jan 23;78(1):10-6. doi: 10.1021/np500429w. Epub 2015 Jan 2.
9
Effects and mechanisms of FBXO31 on Taxol chemoresistance in esophageal squamous cell carcinoma.FBXO31 对食管鳞癌细胞紫杉醇耐药性的影响及其机制。
Biochem Biophys Res Commun. 2022 Jan 1;586:129-136. doi: 10.1016/j.bbrc.2021.11.082. Epub 2021 Nov 24.
10
Insights into anticancer activity and mechanism of action of a ruthenium(II) complex in human esophageal squamous carcinoma EC109 cells.钌（II）配合物对人食管鳞状癌细胞EC109的抗癌活性及作用机制研究
Eur J Pharmacol. 2016 Sep 5;786:60-71. doi: 10.1016/j.ejphar.2016.05.042. Epub 2016 Jun 1.

引用本文的文献

1
Advances and challenges in the treatment of esophageal cancer.食管癌治疗的进展与挑战
Acta Pharm Sin B. 2021 Nov;11(11):3379-3392. doi: 10.1016/j.apsb.2021.03.008. Epub 2021 Mar 9.

本文引用的文献

1
The Antitumor Activity of the Novel Compound Jesridonin on Human Esophageal Carcinoma Cells.新型化合物杰斯里多宁对人食管癌细胞的抗肿瘤活性
PLoS One. 2015 Jun 23;10(6):e0130284. doi: 10.1371/journal.pone.0130284. eCollection 2015.
2
DNA damage, lysosomal degradation and Bcl-xL deamidation in doxycycline- and minocycline-induced cell death in the K562 leukemic cell line.强力霉素和米诺环素诱导K562白血病细胞系细胞死亡过程中的DNA损伤、溶酶体降解及Bcl-xL脱酰胺作用
Biochem Biophys Res Commun. 2015 Jul 31;463(3):268-74. doi: 10.1016/j.bbrc.2015.05.043. Epub 2015 May 27.
3
CIP-36, a novel topoisomerase II-targeting agent, induces the apoptosis of multidrug-resistant cancer cells in vitro.
CIP-36，一种新型拓扑异构酶 II 靶向剂，可诱导体外多药耐药癌细胞凋亡。
Int J Mol Med. 2015 Mar;35(3):771-6. doi: 10.3892/ijmm.2015.2068. Epub 2015 Jan 14.
4
Piperine inhibits proliferation of human osteosarcoma cells via G2/M phase arrest and metastasis by suppressing MMP-2/-9 expression.胡椒碱通过G2/M期阻滞抑制人骨肉瘤细胞增殖，并通过抑制MMP-2/-9表达抑制其转移。
Int Immunopharmacol. 2015 Jan;24(1):50-8. doi: 10.1016/j.intimp.2014.11.012. Epub 2014 Nov 20.
5
Centipedegrass extract induces apoptosis through the activation of caspases and the downregulation of PI3K/Akt and MAPK phosphorylation in leukemia cells.蜈蚣草提取物通过激活半胱天冬酶以及下调白血病细胞中PI3K/Akt和MAPK磷酸化来诱导细胞凋亡。
Int J Mol Med. 2015 Feb;35(2):511-8. doi: 10.3892/ijmm.2014.2012. Epub 2014 Nov 26.
6
ERO1α-dependent endoplasmic reticulum-mitochondrial calcium flux contributes to ER stress and mitochondrial permeabilization by procaspase-activating compound-1 (PAC-1).ERO1α 依赖性内质网-线粒体钙流通过前胱冬酶激活化合物 1（PAC-1）导致内质网应激和线粒体通透性。
Cell Death Dis. 2013 Dec 19;4(12):e968. doi: 10.1038/cddis.2013.502.
7
Autophagy and chemotherapy resistance: a promising therapeutic target for cancer treatment.自噬与化疗耐药：癌症治疗有前景的治疗靶点。
Cell Death Dis. 2013 Oct 10;4(10):e838. doi: 10.1038/cddis.2013.350.
8
Doxorubicin-induced death in tumour cells and cardiomyocytes: is autophagy the key to improving future clinical outcomes?多柔比星诱导肿瘤细胞和心肌细胞死亡：自噬是改善未来临床结局的关键吗？
J Pharm Pharmacol. 2013 Nov;65(11):1577-89. doi: 10.1111/jphp.12144. Epub 2013 Sep 18.
9
Establishment and characterization of a paclitaxel‑resistant human esophageal carcinoma cell line.紫杉醇耐药人食管癌细胞系的建立与鉴定。
Int J Oncol. 2013 Nov;43(5):1607-17. doi: 10.3892/ijo.2013.2083. Epub 2013 Aug 29.
10
Crosstalk between apoptosis, necrosis and autophagy.细胞凋亡、坏死与自噬之间的相互作用。
Biochim Biophys Acta. 2013 Dec;1833(12):3448-3459. doi: 10.1016/j.bbamcr.2013.06.001. Epub 2013 Jun 13.