Bosnjak Masa, Kamensek Urska, Sersa Gregor, Stolfa Danijela, Lavrencak Jaka, Cemazar Maja
Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
Department of Cytopathology, Institute of Oncology Ljubljana, Zaloska 2, 1000, Ljubljana, Slovenia.
J Membr Biol. 2018 Apr;251(2):179-185. doi: 10.1007/s00232-017-9948-z. Epub 2017 Feb 15.
Gene electrotransfer upregulate DNA pattern recognition receptors or DNA sensors, which are part of the innate immune system. In this study, we tested if addition of the cocktail of innate immune system inhibitors to the cells during gene electrotransfer (GET) can increase transfection efficiency and cell survival. The results indicate that this cocktail can decrease cytosolic DNA sensors expression after GET, and consequently increase cell survival and transfection efficiency in B16 cells, but only in highly metastatic B16F10 subtype. We demonstrated that DNA sensors expression during the transfection methods needs to be downregulated if higher transfection efficiency and better cells' survival is needed. The inhibition of the receptors of the innate immune system can improve the transfection efficiency also for GET of malignant melanoma B16 cells, but only of highly metastatic subtype.
基因电穿孔转染可上调作为固有免疫系统一部分的DNA模式识别受体或DNA传感器。在本研究中，我们测试了在基因电穿孔转染（GET）过程中向细胞添加固有免疫系统抑制剂混合物是否能提高转染效率和细胞存活率。结果表明，该混合物可在GET后降低胞质DNA传感器的表达，从而提高B16细胞的存活率和转染效率，但仅在高转移性B16F10亚型中有效。我们证明，如果需要更高的转染效率和更好的细胞存活率，在转染过程中需要下调DNA传感器的表达。抑制固有免疫系统的受体也可提高恶性黑色素瘤B16细胞GET的转染效率，但仅对高转移性亚型有效。
