Yarmush Martin L, Golberg Alexander, Serša Gregor, Kotnik Tadej, Miklavčič Damijan
Center for Engineering in Medicine, Department of Surgery, Massachusetts General Hospital, Harvard Medical School and Shriners Burn Hospital for Children, Boston, Massachusetts 02114; email (M.L.Y.):
Annu Rev Biomed Eng. 2014 Jul 11;16:295-320. doi: 10.1146/annurev-bioeng-071813-104622. Epub 2014 May 27.
When high-amplitude, short-duration pulsed electric fields are applied to cells and tissues, the permeability of the cell membranes and tissue is increased. This increase in permeability is currently explained by the temporary appearance of aqueous pores within the cell membrane, a phenomenon termed electroporation. During the past four decades, advances in fundamental and experimental electroporation research have allowed for the translation of electroporation-based technologies to the clinic. In this review, we describe the theory and current applications of electroporation in medicine and then discuss current challenges in electroporation research and barriers to a more extensive spread of these clinical applications.
当向细胞和组织施加高振幅、短持续时间的脉冲电场时，细胞膜和组织的通透性会增加。目前，这种通透性的增加被解释为细胞膜内出现了临时性的水通道，这一现象被称为电穿孔。在过去的四十年里，基础和实验性电穿孔研究取得的进展使得基于电穿孔的技术得以应用于临床。在这篇综述中，我们描述了电穿孔在医学中的理论和当前应用，然后讨论了电穿孔研究当前面临的挑战以及这些临床应用更广泛推广的障碍。
