Verhoeven J Th J, Koenraadt H M S, Westenberg M, Roenhorst J W
National Plant Protection Organization of the Netherlands, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands.
Naktuinbouw, P.O. Box 40, 2370 AA, Roelofarendsveen, The Netherlands.
Arch Virol. 2017 Jun;162(6):1741-1744. doi: 10.1007/s00705-017-3277-5. Epub 2017 Feb 15.
Tomato apical stunt viroid (TASVd) has been identified in a 24-year old seed lot of Capsicum annuum produced in Taiwan. It is the first finding of TASVd in this plant species. The isolate could be discriminated from all reported isolates of TASVd based on its nucleotide sequence, which showed only 94.8% identity with the most related genotype of TASVd. This discrimination was substantiated by phylogenetic analysis. Inoculation of a RNA extract of contaminated seeds to healthy pepper plants showed that the infectivity of the viroid had remained over time. Nevertheless, no transmission to seedlings was observed.
在台湾生产的一批储存了24年的辣椒种子中发现了番茄顶枯类病毒（TASVd）。这是在该植物物种中首次发现TASVd。根据其核苷酸序列，该分离株可与所有已报道的TASVd分离株区分开来，其与TASVd最相关的基因型仅显示出94.8%的同一性。系统发育分析证实了这种区分。将受污染种子的RNA提取物接种到健康辣椒植株上，结果表明类病毒的感染力随时间持续存在。然而，未观察到向幼苗的传播。
