Razjivin A P, Lukashev E P, Kompanets V O, Kozlovsky V S, Ashikhmin A A, Chekalin S V, Moskalenko A A, Paschenko V Z
Belozersky Research Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia, 119991.
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia, 119991.
Photosynth Res. 2017 Sep;133(1-3):289-295. doi: 10.1007/s11120-017-0341-7. Epub 2017 Feb 16.
Pathways of intramolecular conversion and intermolecular electronic excitation energy transfer (EET) in the photosynthetic apparatus of purple bacteria remain subject to debate. Here we experimentally tested the possibility of EET from the bacteriochlorophyll (BChl) Soret band to the singlet S level of carotenoids using femtosecond pump-probe measurements and steady-state fluorescence excitation and absorption measurements in the near-ultraviolet and visible spectral ranges. The efficiency of EET from the Soret band of BChl to S of the carotenoids in light-harvesting complex LH2 from the purple bacterium Ectothiorhodospira haloalkaliphila appeared not to exceed a few percent.
紫色细菌光合装置中分子内转化和分子间电子激发能量转移（EET）的途径仍存在争议。在此，我们通过飞秒泵浦-探测测量以及近紫外和可见光谱范围内的稳态荧光激发和吸收测量，对从细菌叶绿素（BChl）的索雷特带向类胡萝卜素的单重态S能级进行EET的可能性进行了实验测试。在嗜盐碱外硫红螺菌的捕光复合物LH2中，从BChl的索雷特带向类胡萝卜素的S能级的EET效率似乎不超过百分之几。
