从食品和土壤中分离并鉴定蜡样芽孢杆菌噬菌体
Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil.
作者信息
Oh Hyejin, Seo Dong Joo, Jeon Su Been, Park Hyunkyung, Jeong Suntak, Chun Hyang Sook, Oh Mihwa, Choi Changsun
机构信息
Department of Food and Nutrition, College of Biotechnology and Agriculture Resource, Chung-Ang University, Seodongdaero, Daeduck, Anseong, Gyeonggi, 17546, Republic of Korea.
Department of Food Science and Technology, College of Biotechnology and Agriculture Resource, Chung-Ang University, Anseong, Gyeonggi, 17546, Republic of Korea.
出版信息
Food Environ Virol. 2017 Sep;9(3):260-269. doi: 10.1007/s12560-017-9284-6. Epub 2017 Feb 15.
The aim of this study was to isolate and characterize Bacillus cereus bacteriophages of various origins. Twenty-seven bacteriophages against B. cereus were isolated from various Korean traditional fermented foods and soils. Plaque size, transmission electron microscopy, virulence profile, and in vitro lytic activity of bacteriophage isolates were examined. Transmission electron microscopy confirmed B. cereus bacteriophages belonging to the family Siphoviridae. Among B. cereus bacteriophages with broad host range, 18 isolates (66.7%) did not harbor any B. cereus virulence factors. Among them, bacteriophage strain CAU150036, CAU150038, CAU150058, CAU150064, CAU150065, and CAU150066 effectively inhibited B. cereus in vitro within 1 h. Therefore, they are considered potential candidates for controlling the contamination of B. cereus in food or other applications.
本研究的目的是分离和鉴定不同来源的蜡样芽孢杆菌噬菌体。从韩国各种传统发酵食品和土壤中分离出27株针对蜡样芽孢杆菌的噬菌体。检测了噬菌体分离株的噬菌斑大小、透射电子显微镜观察、毒力谱和体外裂解活性。透射电子显微镜证实蜡样芽孢杆菌噬菌体属于长尾噬菌体科。在具有广泛宿主范围的蜡样芽孢杆菌噬菌体中，18株分离株（66.7%）未携带任何蜡样芽孢杆菌毒力因子。其中，噬菌体菌株CAU150036、CAU150038、CAU150058、CAU150064、CAU150065和CAU150066在1小时内有效抑制体外蜡样芽孢杆菌生长。因此，它们被认为是控制食品中蜡样芽孢杆菌污染或其他应用的潜在候选物。
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