Department of Food and Biotechnology, College of Engineering, Gachon University, Sungnam 461-701, Korea.
J Microbiol Biotechnol. 2012 May;22(5):649-53. doi: 10.4014/jmb.1110.10065.
A temperate phage was isolated from emetic Bacillus cereus NCTC 11143 by mitomycin C and characterized by transmission electron microscopy and DNA and protein analyses. Whole genome sequencing of Bacillus phage 11143 was performed by GS-FLX. The phage has a dsDNA genome of 39,077 bp and a 35% G+C content. Bioinformatic analysis of the phage genome revealed 49 putative ORFs involved in replication, morphogenesis, DNA packaging, lysogeny, and host lysis. Bacillus phage 11143 could be classified as a member of the Siphoviridae family by morphology and genome structure. Genomic comparisons at the DNA and protein levels revealed homologous genetic modules with patterns and morphogenesis proteins similar to those of other Bacillus phages. Thus, Bacillus phages might have a mosaic genetic relationship.
一株温和噬菌体通过丝裂霉素 C 从呕吐型蜡样芽胞杆菌 NCTC 11143 中分离出来，并通过透射电子显微镜和 DNA 及蛋白质分析进行了鉴定。通过 GS-FLX 对蜡样芽胞杆菌噬菌体 11143 进行了全基因组测序。噬菌体具有 39077bp 的双链 DNA 基因组和 35%的 G+C 含量。噬菌体基因组的生物信息学分析显示，有 49 个推定的 ORF 参与复制、形态发生、DNA 包装、溶原和宿主裂解。根据形态和基因组结构，蜡样芽胞杆菌噬菌体 11143 可归类为肌尾噬菌体科的一员。DNA 和蛋白质水平的基因组比较显示，与其他蜡样芽胞杆菌噬菌体具有同源遗传模块，具有相似的形态发生蛋白和模式。因此，蜡样芽胞杆菌噬菌体可能具有镶嵌的遗传关系。
