通过定量质谱法进行甾醇分析。

Sterol Analysis by Quantitative Mass Spectrometry.

作者信息

Jenner Andrew M, Brown Simon H J

机构信息

Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, Australia.

School of Biology and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.

出版信息

Methods Mol Biol. 2017;1583:221-239. doi: 10.1007/978-1-4939-6875-6_17.

DOI:10.1007/978-1-4939-6875-6_17

PMID:28205178

Abstract

Analysis of sterols by mass spectrometry is a fundamental technique allowing for both qualitative and quantitative characterization of sterol molecular lipid species. Lipids are isolated from matrix or matrices by homogenization and solvent extraction, and converted into species amenable for ionization either by derivatization or adduct formation. Chromatogaphy (either gas or liquid phase) can assist with the resolution of sterols. Tandem mass spectrometry allows the precise identification of sterol lipid species, while comparison to internal standards added during extraction enables accurate quantification.

摘要

通过质谱分析甾醇是一项基本技术，可对甾醇分子脂质种类进行定性和定量表征。通过匀浆和溶剂萃取从一种或多种基质中分离脂质，并通过衍生化或加合物形成将其转化为适合电离的种类。色谱法（气相或液相）可辅助甾醇的分离。串联质谱可精确鉴定甾醇脂质种类，而与萃取过程中添加的内标进行比较则可实现准确的定量分析。