甾醇定义系统用于定量研究. 中的甾醇代谢

Faculty of Natural Sciences, University of Haifa, Haifa 3498838 Israel.

STAR Protoc. 2021 Aug 10;2(3):100710. doi: 10.1016/j.xpro.2021.100710. eCollection 2021 Sep 17.

This protocol describes the culturing of the nematode in a sterol-defined experimental system and the subsequent quantitative analysis of sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).

本方案描述了在固醇定义的实验系统中培养线虫，以及随后通过气相色谱-质谱法对固醇进行定量分析的过程。尽管主要在哺乳动物中进行研究，但固醇是大多数真核生物所必需的生物分子。不能合成固醇，因此依赖于摄取膳食固醇。因此，是研究固醇定义条件下代谢的有力系统，本方案对此进行了描述。有关使用和执行本方案的完整详细信息，请参阅 Shamsuzzama 等人（2020 年）。