Yang Alryel, Gelissen Ingrid C
Faculty of Pharmacy, The University of Sydney, Pharmacy Bank Building A15, Camperdown, Sydney, NSW, 2006, Australia.
Methods Mol Biol. 2017;1583:275-285. doi: 10.1007/978-1-4939-6875-6_20.
Cholesterol export from cells to extracellular acceptors represents the first step of the reverse cholesterol transport process and is an essential part of the multifaceted pathway for cells to control their cholesterol levels. Malfunction of this pathway leads to cholesterol accumulation in cells such as macrophages, which can form the basis of conditions like atherosclerosis. A number of ATP-binding cassette (ABC) transporters, namely ABCA1, ABCA7, ABCG1, and ABCG4, play an essential role in this process. In this chapter, we describe methods utilizing radiolabeled sterols for measuring ABC-transporter mediated sterol export, utilizing endogenously expressed transporters as well as overexpression systems.
胆固醇从细胞向细胞外受体的转运是逆向胆固醇转运过程的第一步，也是细胞控制其胆固醇水平的多方面途径的重要组成部分。该途径功能失调会导致胆固醇在巨噬细胞等细胞中积累，这可能成为动脉粥样硬化等病症的基础。一些ATP结合盒（ABC）转运蛋白，即ABCA1、ABCA7、ABCG1和ABCG4，在这一过程中发挥着重要作用。在本章中，我们描述了利用放射性标记固醇来测量ABC转运蛋白介导的固醇转运的方法，这些方法既利用内源性表达的转运蛋白，也利用过表达系统。
