Teixeira Mayza Dalcin, Tureck Luciane Viater, Nascimento Gabrielle Araujo do, Souza Ricardo Lehtonen Rodrigues de, Furtado-Alle Lupe
Universidade Federal do Paraná, Departamento de Genética, Laboratório de Polimorfismos e Ligação, Curitiba, PR, Brazil.
Genet Mol Biol. 2020 Jul 31;43(3):e20190326. doi: 10.1590/1678-4685-GMB-2019-0326.
ATP-Binding Cassette (ABC) transporters are involved in cholesterol metabolism and their dysfunctions could lead to obesity-associated complications. It was investigated whether SNPs in the ABCA1 (rs1800977 and rs2230806), ABCA7 (rs2279796) and ABCG1 (rs692383 and rs3827225) genes can modulate the responsiveness of 137 obese women to a weight-loss dietary intervention. Thus, anthropometric and lipid profiles were collected at baseline and after nine weeks of a calorie-restricted diet of 600kcal per day and participants were genotyped for the ABC genes SNPs. Regarding the transversal analysis, the ABCA7 rs2279796 GG genotype was associated with higher levels of total cholesterol and LDL-c at baseline (p = 0.044 for both). Association between ABCG1 rs692383 AA genotype and lower BMI were found in the post-diet moment, however, statistical significance was lost after multi-test correction. Regarding the longitudinal analysis, after multi-test correction, the association remained between ABCG1 rs692383 G allele and HDL-c levels: G allele carriers had a lower HDL-c reduction (p = 0.043). Results suggest the standard weight-loss diet applied in this study could attenuate the ABCA7 rs2279796 GG genotype effects found at baseline and non-dyslipidemic obese women with ABCG1 rs692383 G allele are benefitting from the diet with a lower reduction in HDL-c levels.
ATP结合盒（ABC）转运蛋白参与胆固醇代谢，其功能障碍可能导致肥胖相关并发症。本研究旨在探讨ABCA1基因（rs1800977和rs2230806）、ABCA7基因（rs2279796）和ABCG1基因（rs692383和rs3827225）中的单核苷酸多态性（SNPs）是否能调节137名肥胖女性对减肥饮食干预的反应。因此，在基线时以及在每天600千卡热量限制饮食9周后收集人体测量数据和血脂谱，并对参与者的ABC基因SNPs进行基因分型。横向分析方面，ABCA7 rs2279796 GG基因型与基线时较高的总胆固醇和低密度脂蛋白胆固醇水平相关（两者p = 0.044）。在饮食后阶段发现ABCG1 rs692383 AA基因型与较低的体重指数之间存在关联，然而，在多重检验校正后统计学意义消失。纵向分析方面，在多重检验校正后，ABCG1 rs692383 G等位基因与高密度脂蛋白胆固醇水平之间的关联仍然存在：G等位基因携带者的高密度脂蛋白胆固醇降低幅度较小（p = 0.043）。结果表明，本研究中应用的标准减肥饮食可以减弱基线时发现的ABCA7 rs2279796 GG基因型的影响，并且携带ABCG1 rs692383 G等位基因的非血脂异常肥胖女性从该饮食中受益，其高密度脂蛋白胆固醇水平降低幅度较小。
