Kim Wi-Sik, Kong Kyoung-Hui, Kim Jong-Oh, Jung Sung-Ju, Kim Jeong-Ho, Oh Myung-Joo
Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University, Yeosu, Korea (W-S Kim, Kong, J-O Kim, Jung).
Faculty of Marine Bioscience and Technology, Gangneung-Wonju National University, Gangneung, Korea (J-H Kim).
J Vet Diagn Invest. 2017 May;29(3):357-361. doi: 10.1177/1040638717690783. Epub 2017 Feb 16.
In 2015, 6.7-60% mortality was observed in black seabream ( Acanthopagrus schlegelii), rock bream ( Oplegnathus fasciatus), and gray mullet ( Mugil cephalus) farmed in the southern coast of Korea. On examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. Some rock bream and gray mullet were coinfected with bacteria ( Pseudomonas anguilliseptica, Vibrio tapetis, or Vibrio anguillarum). Histologic examination revealed extensive hyperplastic epithelium and lamellar fusion in the gills. Numerous amoebae were seen between gill filaments. The amoebae collected from the 3 fish species had specific 630 bp of a partial 18S rRNA gene fragment for Neoparamoeba perurans. Phylogenetic analysis based on partial 18S rRNA gene nucleotide sequences revealed that these Korean amoeba isolates belonged to the N. perurans group. Based on our results, black seabream, rock bream, and gray mullet were added as new hosts for N. perurans.
2015年，在韩国南部海岸养殖的黑鲷（黑棘鲷）、条石鲷和鲻鱼中观察到6.7%至60%的死亡率。经检查，在这三种鱼类的鳃上发现了大量变形虫，检出率为100%。一些条石鲷和鲻鱼同时感染了细菌（鳗弧菌、塔氏弧菌或鳗鲡弧菌）。组织学检查显示鳃中有广泛的上皮增生和鳃小片融合。在鳃丝之间可见大量变形虫。从这三种鱼类收集的变形虫具有针对秘鲁新变形虫的18S rRNA基因部分片段的特定630 bp。基于18S rRNA基因部分核苷酸序列的系统发育分析表明，这些韩国变形虫分离株属于秘鲁新变形虫组。根据我们的结果，黑鲷、条石鲷和鲻鱼被添加为秘鲁新变形虫的新宿主。
