Kelly J, O'Farrelly C, O'Mahony C, Weir D G, Feighery C
Department of Immunology, St James's Hospital, Dublin.
Clin Exp Immunol. 1987 Apr;68(1):177-88.
Immunohistological analysis of the cellular composition of the small intestinal mucosa in a group of untreated and treated coeliac patients and non-coeliac control subjects was performed using monoclonal antibodies and an immunoperoxidase technique. A characteristic cellular distribution was observed within the normal mucosa. The intraepithelial and lamina propria compartments were occupied mainly by T suppressor/cytotoxic and T helper/inducer cells respectively. Further subdivision of lamina propria T helper/inducer cells with the Leu 8 antibody revealed that these were of the Leu 3a+ Leu 8- phenotype. Macrophages, defined by the RFD7 antibody, were seen to occupy the same microenvironment as T helper/inducer cells. T cells expressing the T cell activation antigen defined by anti-Ta1 were found with the normal lamina propria, although few cells were identified by the anti-Tac antibody. HLA-Dr antigens were expressed by stellate cells within the lamina propria, and also by the epithelial cells of the villi, but not by normal crypt epithelial cells. In untreated coeliac patients the distribution of the various cell types was essentially unchanged, although the number of these cells was markedly increased, including those which expressed the Ta1 antigen. A significant deviation from normal in the expression of HLA-DR antigens was found in the coeliac small bowel: these antigens were expressed not only on the villous epithelial cells but also on the epithelial cells of the crypts. Immunohistological findings in the treated coeliac patients were intermediate between the normal and untreated coeliac groups, and were completely normal in those patients with complete histological resolution of their disease. These results suggest that coeliac disease is accompanied by an enhanced stimulation of the normal mucosal immune response and do not imply a primary pathogenic role for the immune system in this disease.
采用单克隆抗体和免疫过氧化物酶技术，对一组未经治疗和经过治疗的乳糜泻患者以及非乳糜泻对照受试者的小肠黏膜细胞组成进行了免疫组织学分析。在正常黏膜内观察到了特征性的细胞分布。上皮内和固有层区域分别主要由抑制性/细胞毒性T细胞和辅助性/诱导性T细胞占据。用Leu 8抗体对固有层辅助性/诱导性T细胞进一步细分显示，这些细胞具有Leu 3a + Leu 8 - 表型。由RFD7抗体界定的巨噬细胞与辅助性/诱导性T细胞占据相同的微环境。在正常固有层中发现了表达由抗Ta1界定的T细胞活化抗原的T细胞，尽管用抗Tac抗体鉴定出的细胞很少。HLA - Dr抗原由固有层内的星状细胞以及绒毛的上皮细胞表达，但正常隐窝上皮细胞不表达。在未经治疗的乳糜泻患者中，尽管这些细胞的数量显著增加，包括那些表达Ta1抗原的细胞，但各种细胞类型的分布基本未变。在乳糜泻小肠中发现HLA - Dr抗原的表达与正常情况有显著差异：这些抗原不仅在绒毛上皮细胞上表达，也在隐窝的上皮细胞上表达。经治疗的乳糜泻患者的免疫组织学结果介于正常组和未经治疗的乳糜泻组之间，而疾病组织学完全缓解的患者结果完全正常。这些结果表明，乳糜泻伴随着正常黏膜免疫反应的增强刺激，并不意味着免疫系统在该疾病中起主要致病作用。
