Marley N J, Macartney J C, Ciclitira P J
Department of Histopathology, Rayne Institute, St Thomas' Hospital, London, UK.
Clin Exp Immunol. 1987 Nov;70(2):386-93.
Frozen sections of jejunal mucosa from control subjects and patients with both treated and untreated coeliac disease were examined for HLA class II DR, DP and DQ expression. Different staining patterns with monoclonal antibodies to the different class II subgroups were observed with the control subjects. There was some inter-subject variation but in general DR greater than DP greater than DQ staining was observed with the villous enterocytes staining most strongly with the staining decreasing towards the crypt bases. The patients with treated coeliac disease gave a similar pattern to the controls. The patients with untreated coeliac disease generally gave a more intense and relatively uniform staining of both surface and crypt enterocytes for all class II subgroups.
对来自对照受试者以及患有已治疗和未治疗乳糜泻患者的空肠黏膜冰冻切片进行检查，以检测HLA II类DR、DP和DQ的表达。在对照受试者中观察到针对不同II类亚组的单克隆抗体呈现不同的染色模式。受试者之间存在一些差异，但总体上观察到DR染色强于DP染色，DP染色强于DQ染色，绒毛肠上皮细胞染色最强，向隐窝底部染色逐渐减弱。已治疗乳糜泻的患者呈现出与对照相似的模式。未治疗乳糜泻的患者，其所有II类亚组的表面和隐窝肠上皮细胞通常都有更强且相对均匀的染色。
