Holm K, Savilahti E, Koskimies S, Lipsanen V, Mäki M
Department of Clinical Sciences, University of Tampere, Finland.
Gut. 1994 Jan;35(1):55-60. doi: 10.1136/gut.35.1.55.
The staining of HLA class II antigens, the presence of cells positive for interleukin-2 receptors, the proportion of crypt cells in mitosis in the jejunal biopsy specimens, and the dose of coeliac disease marker HLA-DQ genes were studied in 75 healthy family members of coeliac disease patients. Eleven had silent coeliac disease; in the rest the morphology of the jejunum was normal. In the specimens from family members, staining of epithelial cells with HLA-DP and -DR antibodies was more widely distributed and stronger than in those from 19 controls. Interleukin-2 receptor+ cells were seen in the epithelium of all eight specimens from subjects with silent coeliac disease, and also in 24 morphologically normal specimens from family members, but not in the 19 control specimens. The proportion of crypt cells in mitosis was increased only in the specimens from the subjects with silent coeliac disease. The staining intensity of the epithelial cells with HLA-DP and -DR antibodies, the presence of interleukin-2 receptor+ cells and the percentage of crypt cells in mitosis were significantly associated with the number of coeliac disease marker DQB genes. Many family members of patients with coeliac disease have signs of inflammation even in morphologically normal jejunum; these inflammatory changes together with coeliac disease marker DQ genes may point to latent disease in these subjects.
对75名乳糜泻患者的健康家庭成员进行了研究，检测了他们HLA II类抗原的染色情况、白细胞介素-2受体阳性细胞的存在情况、空肠活检标本中有丝分裂的隐窝细胞比例以及乳糜泻标志物HLA-DQ基因的剂量。其中11人患有无症状性乳糜泻；其余人的空肠形态正常。在家庭成员的标本中，与19名对照者的标本相比，用HLA-DP和-DR抗体对上皮细胞进行染色时，分布更广泛且染色更强。在所有8例无症状性乳糜泻患者的标本上皮中均可见白细胞介素-2受体阳性细胞，在24例家庭成员的形态正常标本中也可见到，但在19例对照标本中未见到。仅在无症状性乳糜泻患者的标本中有丝分裂的隐窝细胞比例增加。上皮细胞用HLA-DP和-DR抗体的染色强度、白细胞介素-2受体阳性细胞的存在情况以及有丝分裂的隐窝细胞百分比与乳糜泻标志物DQB基因的数量显著相关。许多乳糜泻患者的家庭成员即使在形态正常的空肠中也有炎症迹象；这些炎症变化以及乳糜泻标志物DQ基因可能表明这些受试者存在潜在疾病。
