利用多功能酰基转移酶KirCII进行聚酮化合物的生物衍生化
Polyketide Bioderivatization Using the Promiscuous Acyltransferase KirCII.
作者信息
Musiol-Kroll Ewa M, Zubeil Florian, Schafhauser Thomas, Härtner Thomas, Kulik Andreas, McArthur John, Koryakina Irina, Wohlleben Wolfgang, Grond Stephanie, Williams Gavin J, Lee Sang Yup, Weber Tilmann
机构信息
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark , Kemitorvet Building B220, 2800 Kgs. Lyngby, Denmark.
German Centre for Infection Research (DZIF), Partner site Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
出版信息
ACS Synth Biol. 2017 Mar 17;6(3):421-427. doi: 10.1021/acssynbio.6b00341. Epub 2017 Feb 22.
Abstract
During polyketide biosynthesis, acyltransferases (ATs) are the essential gatekeepers which provide the assembly lines with precursors and thus contribute greatly to structural diversity. Previously, we demonstrated that the discrete AT KirCII from the kirromycin antibiotic pathway accesses nonmalonate extender units. Here, we exploit the promiscuity of KirCII to generate new kirromycins with allyl- and propargyl-side chains in vivo, the latter were utilized as educts for further modification by "click" chemistry.
摘要
在聚酮生物合成过程中，酰基转移酶（ATs）是至关重要的守门人，它们为装配线提供前体，从而对结构多样性有很大贡献。此前，我们证明了来自奇霉素抗生素途径的离散型AT KirCII能够利用非丙二酸延伸单元。在此，我们利用KirCII的底物选择性来在体内生成带有烯丙基和炔丙基侧链的新奇霉素，后者被用作通过“点击”化学进行进一步修饰的反应物。
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本文引用的文献
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