Ploos van Amstel H K, van der Zanden A L, Reitsma P H, Bertina R M
Dept of Hematology, Leiden University Hospital, The Netherlands.
FEBS Lett. 1987 Sep 28;222(1):186-90. doi: 10.1016/0014-5793(87)80217-x.
Partial cDNAs coding for human protein S were isolated from a pUC9 human liver cDNA library. Together, the overlapping clones span a (partial) 5'-non-coding region, and the complete protein S coding and 3'-untranslated regions. The derived amino acid sequence deviates at five positions from two previously reported protein S sequences. Two of these differences (Phe instead of Leu at position -16 and Tyr instead of Asp at position 222) are found in regions that are important for the post-translational modification of protein S, the gamma-carboxylation of glutamic acid and the hydroxylation of asparagine, respectively.
编码人蛋白S的部分cDNA是从一个pUC9人肝脏cDNA文库中分离出来的。这些重叠克隆共同跨越一个（部分）5'-非编码区、完整的蛋白S编码区和3'-非翻译区。推导的氨基酸序列在五个位置上与先前报道的两个蛋白S序列不同。其中两个差异（-16位的苯丙氨酸取代亮氨酸和222位的酪氨酸取代天冬氨酸）分别出现在对蛋白S翻译后修饰、谷氨酸的γ-羧化和天冬酰胺的羟基化很重要的区域。
