与人类蛋白S cDNA同源的两个基因位于3号染色体上。
Two genes homologous with human protein S cDNA are located on chromosome 3.
作者信息
Ploos van Amstel J K, van der Zanden A L, Bakker E, Reitsma P H, Bertina R M
机构信息
Department of Hematology, Leiden University Hospital, The Netherlands.
出版信息
Thromb Haemost. 1987 Dec 18;58(4):982-7.
A cDNA coding for the carboxy-terminal region of human protein S and containing a complete 3'-untranslated region, was isolated by a combination of antibody screening of a lambda gt11 human liver cDNA expression library and in situ hybridization of a pUC9 human liver cDNA library. Hybridization analysis of human total DNA with radiolabelled non-overlapping cDNA restriction fragments revealed the existence of two genes per haploid genome homologous with the protein S cDNA. Both genes were mapped to chromosome 3 using human-rodent cell hybrids. Neither of the genes showed polymorphism for sixteen different enzymes upon hybridization with the protein S cDNA.
通过对λgt11人肝cDNA表达文库进行抗体筛选和对pUC9人肝cDNA文库进行原位杂交相结合的方法，分离出了一个编码人蛋白S羧基末端区域且包含完整3'-非翻译区的cDNA。用人总DNA与放射性标记的非重叠cDNA限制性片段进行杂交分析，结果显示每个单倍体基因组存在两个与蛋白S cDNA同源的基因。利用人-啮齿动物细胞杂种将这两个基因都定位到了3号染色体上。与蛋白S cDNA杂交时，这两个基因在16种不同的酶切反应中均未显示出多态性。
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