Hartlieb Matthias, Bus Tanja, Kübel Joachim, Pretzel David, Hoeppener Stephanie, Leiske Meike N, Kempe Kristian, Dietzek Benjamin, Schubert Ulrich S
Laboratory of Organic and Macromolecular Chemistry (IOMC), Friedrich Schiller University Jena , Humboldtstrasse 10, 07743, Jena, Germany.
Jena Center for Soft Matter (JCSM), Friedrich Schiller University Jena , Philosophenweg 7, 07743, Jena, Germany.
Bioconjug Chem. 2017 Apr 19;28(4):1229-1235. doi: 10.1021/acs.bioconjchem.7b00067. Epub 2017 Mar 3.
Controlling the size and charge of nanometer-sized objects is of upmost importance for their interactions with cells. We herein present the synthesis of poly(2-oxazoline) based nanogels comprising a hydrophilic shell and an amine containing core compartment. Amine groups were cross-linked using glutaraldehyde resulting in imine based nanogels. As a drug model, amino fluorescein was covalently immobilized within the core, quenching excessive aldehyde functions. By varying the amount of cross-linker, the zeta potential and, hence, the cellular uptake could be adjusted. The fluorescence of the nanogels was found to be dependent on the cross-linking density. Finally, the hemocompatibility of the described systems was studied by hemolysis and erythrocyte aggregation assays. While cellular uptake was shown to be dependent on the zeta potential of the nanogel, no harmful effects to red blood cells was observed, rendering the present system as an interesting toolbox for the production of nanomaterials with a defined biological interaction profile.
控制纳米级物体的大小和电荷对于它们与细胞的相互作用至关重要。我们在此展示了基于聚（2-恶唑啉）的纳米凝胶的合成，该纳米凝胶包含一个亲水壳层和一个含胺的核心隔室。使用戊二醛交联胺基，得到基于亚胺的纳米凝胶。作为药物模型，氨基荧光素被共价固定在核心内，淬灭过量的醛功能。通过改变交联剂的量，可以调节zeta电位，从而调节细胞摄取。发现纳米凝胶的荧光取决于交联密度。最后，通过溶血和红细胞聚集试验研究了所述系统的血液相容性。虽然细胞摄取显示取决于纳米凝胶的zeta电位，但未观察到对红细胞的有害影响，这使得本系统成为生产具有明确生物相互作用特征的纳米材料的有趣工具箱。
