Legros Camille, Wirotius Anne-Laure, De Pauw-Gillet Marie-Claire, Tam Kam Chiu, Taton Daniel, Lecommandoux Sébastien
Université de Bordeaux , UMR5629, ENSCPB, 16 Avenue Pey Berland, 33607 Pessac Cedex, France.
Biomacromolecules. 2015 Jan 12;16(1):183-91. doi: 10.1021/bm501393q. Epub 2014 Nov 26.
Hydrophilic nanogels based on partially hydrolyzed poly(2-ethyl-2-oxazoline) were synthesized in dilute aqueous media in the presence of 1,6-hexanediol diglycidyl ether as a cross-linker. Nanogel formation was monitored by DLS and HSQC NMR spectroscopy, and the final nano-objects were characterized by DLS, TEM, AFM, and NanoSight analyses. Nanogels with a hydrodynamic radius of 78 nm exhibiting a slight positive surface charge were obtained. MTS assays (cell metabolic activity test) evidenced that nanogels were nontoxic in the investigated concentration range (i.e., 0.1 to 400 μg/mL) and that no specific interaction with bovine serum albumin was observed.
以1,6 -己二醇二缩水甘油醚作为交联剂，在稀水介质中合成了基于部分水解聚（2 -乙基- 2 -恶唑啉）的亲水性纳米凝胶。通过动态光散射（DLS）和异核单量子相干（HSQC）核磁共振光谱监测纳米凝胶的形成，并用DLS、透射电子显微镜（TEM）、原子力显微镜（AFM）和纳米可视分析对最终的纳米物体进行表征。获得了流体力学半径为78 nm且表面带有轻微正电荷的纳米凝胶。噻唑蓝（MTS）法（细胞代谢活性测试）证明，在所研究的浓度范围（即0.1至400 μg/mL）内纳米凝胶无毒，且未观察到与牛血清白蛋白的特异性相互作用。
