Laurent E, Mockel J, Van Sande J, Graff I, Dumont J E
Institute of Interdisciplinary Research, School of Medicine, Hospital Erasme, Brussels, Belgium.
Mol Cell Endocrinol. 1987 Aug;52(3):273-8. doi: 10.1016/0303-7207(87)90055-4.
In human thyroid slices prelabeled with myo-[2-3H]inositol, thyrotropin (TSH, 3-30 mU/ml) stimulated IP3, IP2 and IP1 generation over a prolonged time course. The cAMP response was much more sensitive to TSH, peaking between 1 and 5 mU/ml. Forskolin (10(-5) M) and isoproterenol had no effect on basal IP levels, while carbamylcholine (10(-5) M, 10(-4) M) also increased IP accumulation. These data suggest that in the human thyroid, TSH activates a phospholipase C generating IP3 and diacylglycerol independently of the well-known adenylate cyclase stimulation. They validate in the human model a dual mode of action of the hormone previously proposed on the basis of indirect observations.
在用肌醇-[2-³H]预先标记的人甲状腺切片中，促甲状腺激素（TSH，3 - 30 mU/ml）在较长时间内刺激了IP3、IP2和IP1的生成。cAMP反应对TSH更为敏感，在1至5 mU/ml之间达到峰值。福斯高林（10⁻⁵ M）和异丙肾上腺素对基础IP水平无影响，而氨甲酰胆碱（10⁻⁵ M，10⁻⁴ M）也增加了IP的积累。这些数据表明，在人甲状腺中，TSH激活磷脂酶C生成IP3和二酰甘油，与众所周知的腺苷酸环化酶刺激无关。它们在人体模型中验证了先前基于间接观察提出的激素双重作用模式。
