Department of Chemistry, Universidad de Burgos , Plaza Misael Bañuelos s/n, E-09001 Burgos, Spain.
Anal Chem. 2017 Feb 7;89(3):1815-1822. doi: 10.1021/acs.analchem.6b04155. Epub 2017 Jan 24.
The study of real samples is more complicated than the study of other systems. However, the inherent advantages of UV-vis absorption spectroelectrochemistry should overcome some difficulties related to direct measurements in complex matrices. For this reason, a singular spectroelectrochemistry device has been fabricated and validated. The novel cell is based on single-walled carbon nanotubes, which are filtered and subsequently press-transferred on a polyethylene terephthalate support using a stencil with a custom design. With this new methodology, working, counter, and reference electrodes are completely flat on the surface, where two optical fibers are fixed in a long optical path length configuration. To demonstrate the usefulness of this device and the power of spectroelectrochemistry techniques to solve problems of the current world, this device is used to quantitatively detect the concentration of ascorbic acid in a complex matrix such as a fruit, directly, without any previous sample pretreatment. The ease to fabricate the device, the advantages related to its use, and the excellent results obtained not only with univariate but also with multivariate analysis, shed more light on the analysis of samples as they occur in nature. According to the particular features of this cell, to the best of our knowledge this is the first spectroelectrochemical sensor that can be inserted directly in a biological matrix, laying the groundwork to perform in vivo measurements in a near future.
研究实际样本比研究其他系统更为复杂。然而，紫外可见吸收光谱电化学的固有优势应该能够克服与在复杂基质中直接测量相关的一些困难。出于这个原因，我们已经制造并验证了一种独特的光谱电化学装置。该新型电池基于单壁碳纳米管，通过使用具有定制设计的模板进行过滤和随后的压印转移到聚对苯二甲酸乙二醇酯载体上。使用这种新方法，工作电极、对电极和参比电极完全在表面上是平坦的，其中两根光纤固定在长光程配置中。为了展示该装置的实用性以及光谱电化学技术解决当前世界问题的能力，该装置用于直接定量检测水果等复杂基质中抗坏血酸的浓度，无需任何先前的样品预处理。该装置易于制造，使用相关的优势，以及不仅在单变量分析而且在多变量分析中获得的出色结果，使我们更深入地了解了自然发生的样品分析。根据该电池的特殊特征，据我们所知，这是第一个可以直接插入生物基质中的光谱电化学传感器，为不久的将来进行体内测量奠定了基础。
