State Key Laboratory of Applied Organic Chemistry, Lanzhou University , Lanzhou 730000, People's Republic of China.
Key Laboratory of Salt Lakes Resources and Chemistry, Qinghai Institute of Salt Lakes, Chinese Academy of Sciences , Xining 810008, People's Republic of China.
Anal Chem. 2017 Feb 7;89(3):1937-1944. doi: 10.1021/acs.analchem.6b04431. Epub 2017 Jan 6.
A dual mode fluorescent probe, which is based on an integration of fluorescein and coumarin fluorophores, was developed for the discrimination of Cys from Hcy and GSH. This probe (2) shows the advantage of quick reaction (5 min) with Cys, resulting in a strong fluorescence turn-on response when excited at 450 nm. Notably, it also demonstrates the ratiometric fluorescence property while excited by a shorter wavelength (332 nm). All of results suggest probe 2 has a high selectivity toward Cys even in the presence of other amino acids, cations and anions. The detection limit of Cys was calculated as 0.084 μM, which was much lower than the intracellular concentration. H NMR, MS and DFT calculation were used to reveal the detection mechanism further. Finally, this low cytotoxic probe was successfully applied in bioimaging within HepG2 cells.
一种双模式荧光探针，基于荧光素和香豆素荧光团的整合，被开发用于区分半胱氨酸（Cys）、同型半胱氨酸（Hcy）和谷胱甘肽（GSH）。该探针（2）具有与 Cys 快速反应（5 分钟）的优势，在 450nm 激发时产生强烈的荧光开启响应。值得注意的是，当用较短波长（332nm）激发时，它还表现出比率荧光性质。所有结果表明，探针 2 对 Cys 具有很高的选择性，即使在存在其他氨基酸、阳离子和阴离子的情况下也是如此。Cys 的检测限计算为 0.084 μM，远低于细胞内浓度。1 H NMR、MS 和 DFT 计算进一步揭示了检测机制。最后，这种低细胞毒性探针成功地应用于 HepG2 细胞内的生物成像。
