基于荧光香豆素的探针用于快速检测半胱氨酸及其在活细胞中的应用。

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Molecules. 2017 Sep 26;22(10):1618. doi: 10.3390/molecules22101618.

A new coumarin-based fluorescent probe, containing an allylic esters group, has been designed and synthesized for sensing cysteine in physiological pH. In this fluorescent probe, the coumarin was applied as the fluorophore and an allylic esters group was combined as both a fluorescence quencher and a recognition unit. The probe can selectively and sensitively detect cysteine (Cys) over homocysteine, glutathione, and other amino acids, and has a rapid response time of 30 min and a low detection limit of 47.7 nM. In addition, the probe could be applied for cell imaging with low cytotoxicity.

一种新型的基于香豆素的荧光探针，含有烯丙基酯基团，已被设计和合成用于在生理 pH 值下检测半胱氨酸。在这个荧光探针中，香豆素被用作荧光团，烯丙基酯基团既作为荧光猝灭剂又作为识别单元。该探针可以选择性和灵敏地检测半胱氨酸（Cys），而对高半胱氨酸、谷胱甘肽和其他氨基酸没有响应，具有 30 分钟的快速响应时间和 47.7 nM 的低检测限。此外，该探针可用于具有低细胞毒性的细胞成像。