Kaur C, Ling E A, Wong W C
Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge.
J Anat. 1987 Jun;152:13-22.
The activity of TPPase in amoeboid microglial cells has been studied in postnatal rats. When examined with the light microscope such cells in 1-10 days old rats perfused with 4% paraformaldehyde were round and showed a dark brown reaction in their cytoplasm. In older rats (10-30 days), the reactive amoeboid microglial cells were oval, flattened or branched. Electron microscopic examination revealed that the reaction product was seen on the plasma membrane, in the subplasmalemmal vacuoles, in tubular invaginations of plasma membrane and in the transface of the Golgi saccules. In rats perfused with the mixed aldehyde solution, the amoeboid microglial cells did not show a positive TPPase reaction with the light microscope but at the ultrastructural level a weak reaction was seen in some cytoplasmic vacuoles and in the Golgi saccules.
已对新生大鼠中阿米巴样小胶质细胞的TPP酶活性进行了研究。在用4%多聚甲醛灌注的1至10日龄大鼠中，用光学显微镜检查时，此类细胞呈圆形，其细胞质呈现深棕色反应。在较大的大鼠（10至30日龄）中，有反应的阿米巴样小胶质细胞呈椭圆形、扁平状或有分支。电子显微镜检查显示，反应产物可见于质膜、质膜下液泡、质膜的管状内陷以及高尔基体囊泡的反面。在用混合醛溶液灌注的大鼠中，阿米巴样小胶质细胞在光学显微镜下未显示TPP酶阳性反应，但在超微结构水平上，在一些细胞质液泡和高尔基体囊泡中可见微弱反应。
