Nies D, Mergeay M, Friedrich B, Schlegel H G
Institut für Pflanzenphysiologie, Zellbiologie und Mikrobiologie, Freien Universität Berlin, Federal Republic of Germany.
J Bacteriol. 1987 Oct;169(10):4865-8. doi: 10.1128/jb.169.10.4865-4868.1987.
A 238-kilobase-pair plasmid, pMOL30, confers resistance to cadmium, zinc, and cobalt salts in Alcaligenes eutrophus CH34. After Tn5 mutagenesis, restriction nuclease analysis, and Southern DNA-DNA hybridization, a 9.1-kilobase-pair EcoRI fragment was found to harbor all of these resistance properties and was cloned into the broad-host-range hybrid plasmid pRK290. When transferred to a plasmid-free derivative of CH34, the hybrid plasmid conferred the same degree of resistance as the parent plasmid pMOL30. In two other Alcaligenes strains, the hybrid plasmid was expressed, but to a lower degree than in CH34 derivatives.
一个238千碱基对的质粒pMOL30，赋予嗜碱假单胞菌CH34对镉盐、锌盐和钴盐的抗性。经Tn5诱变、限制性核酸酶分析和Southern DNA-DNA杂交后，发现一个9.1千碱基对的EcoRI片段携带所有这些抗性特性，并被克隆到广宿主范围的杂交质粒pRK290中。当转移到CH34的无质粒衍生物中时，该杂交质粒赋予的抗性程度与亲本质粒pMOL30相同。在另外两种嗜碱假单胞菌菌株中，该杂交质粒也能表达，但表达程度低于在CH34衍生物中的表达程度。
