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木糖氧化产碱杆菌31A的ncc基因座编码的镍钴镉联合抗性。

Combined nickel-cobalt-cadmium resistance encoded by the ncc locus of Alcaligenes xylosoxidans 31A.

作者信息

Schmidt T, Schlegel H G

机构信息

Institut für Mikrobiologie, Georg-August-Universität, Göttingen, Germany.

出版信息

J Bacteriol. 1994 Nov;176(22):7045-54. doi: 10.1128/jb.176.22.7045-7054.1994.

DOI:10.1128/jb.176.22.7045-7054.1994
PMID:7961470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC197079/
Abstract

The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt, and cadmium resistance in strain AE104, which is a cured derivative of the metal-resistant bacterium Alcaligenes eutrophus CH34. ncc is not expressed in Escherichia coli. The nre locus causes low-level nickel resistance in both Alcaligenes and E. coli strains. The nucleotide sequence of the ncc locus revealed seven open reading frames designated nccYXHCBAN. The corresponding predicted proteins share strong similarities with proteins encoded by the metal resistance loci cnr (cnrYXHCBA) and czc (czcRCBAD) of A. eutrophus CH34. When different DNA fragments carrying ncc genes were heterologously expressed under the control of the bacteriophage T7 promoter, five protein bands representing NccA (116 kDa), NccB (40 kDa), NccC (46 kDa), NccN (23.5 kDa), and NccX (16.5 kDa) were detected.

摘要

木糖氧化产碱菌31A的质粒pTOM9携带的镍 - 钴 - 镉抗性基因位于一个14.5kb的BamHI片段上。通过随机Tn5插入诱变，该片段被证明含有两个不同的镍抗性位点，即ncc和nre。ncc位点在菌株AE104中引起高水平的镍、钴和镉联合抗性，AE104是金属抗性细菌嗜碱假单胞菌CH34的一个治愈衍生物。ncc在大肠杆菌中不表达。nre位点在产碱菌和大肠杆菌菌株中均引起低水平的镍抗性。ncc位点的核苷酸序列揭示了七个开放阅读框，命名为nccYXHCBAN。相应的预测蛋白与嗜碱假单胞菌CH34的金属抗性位点cnr（cnrYXHCBA）和czc（czcRCBAD）编码的蛋白具有很强的相似性。当携带ncc基因的不同DNA片段在噬菌体T7启动子的控制下进行异源表达时，检测到五条代表NccA（116 kDa）、NccB（40 kDa）、NccC（46 kDa）、NccN（23.5 kDa）和NccX（16.5 kDa）的蛋白条带。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e789/197079/823ae15e04b2/jbacter00040-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e789/197079/823ae15e04b2/jbacter00040-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e789/197079/823ae15e04b2/jbacter00040-0278-a.jpg

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