Soll D R, Langtimm C J, McDowell J, Hicks J, Galask R
Department of Biology, University of Iowa, Iowa City 52242.
J Clin Microbiol. 1987 Sep;25(9):1611-22. doi: 10.1128/jcm.25.9.1611-1622.1987.
High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it was demonstrated that 4 of the 11 isolates contained multiple-switch phenotypes at the site of infection and that 9 of the 11 isolates were in a high-frequency mode of switching (10(-2) to 10(-3)). Isolates could be separated into four general categories of switching repertoires. To demonstrate that multiple phenotypes at the site of a single infection represented the same strain, EcoRI digests of total cell DNA were separated on agarose gels, and Southern hybridization patterns with two cloned midrepeat sequences were compared.
对11例急性白色念珠菌阴道炎患者感染部位的高频转换和菌株变异性进行了评估。通过直接从感染部位克隆细胞，证明11株分离株中有4株在感染部位含有多种转换表型，11株分离株中有9株处于高频转换模式（10^(-2)至10^(-3)）。分离株可分为四种一般类型的转换谱。为了证明单一感染部位的多种表型代表同一菌株，将总细胞DNA的EcoRI酶切产物在琼脂糖凝胶上分离，并比较了两个克隆的中间重复序列的Southern杂交模式。
