Whelan W L, Kirsch D R, Kwon-Chung K J, Wahl S M, Smith P D
Cellular Immunology Section, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
J Infect Dis. 1990 Aug;162(2):513-8. doi: 10.1093/infdis/162.2.513.
To determine whether Candida albicans in patients with AIDS represents a unique strain, C. albicans isolated from 24 patients with AIDS was compared with Candida isolated from 23 healthy adults. Resistance to 5-fluorocytosine, synthesis of amino acids and nucleotides, sugar use, and enzyme activity patterns were similar among isolates from the two groups. Molecular analysis revealed similar banding patterns of EcoRI restriction fragments of DNA between 2.5-3 and 6-7 kb. In addition, the frequency of a dimorphic 3.7-versus 4.2-kb band, identified by agarose gel electrophoresis and by probing Southern transfers of EcoRI digests with a cloned fragment of C. albicans DNA encoding 25S ribosomal RNA, was not significantly different between the AIDS-derived and control C. albicans. C. albicans isolated at different time points in the course of disease and from different sites in individual patients showed identical DNA fingerprints. The similarity in isolates of C. albicans that cause disease in AIDS patients and those present in healthy subjects suggests that the candidiasis associated with AIDS is not due to the presence of a unique or particularly virulent strain but is likely the consequence of a defect in host defense mechanisms.
为了确定艾滋病患者体内的白色念珠菌是否代表一种独特的菌株，将从24例艾滋病患者中分离出的白色念珠菌与从23名健康成年人中分离出的念珠菌进行了比较。两组分离株对5-氟胞嘧啶的耐药性、氨基酸和核苷酸的合成、糖的利用以及酶活性模式相似。分子分析显示，在2.5至3 kb和6至7 kb之间的DNA的EcoRI限制性片段具有相似的条带模式。此外，通过琼脂糖凝胶电泳以及用编码25S核糖体RNA的白色念珠菌DNA克隆片段探测EcoRI消化产物的Southern印迹转移所鉴定的3.7 kb与4.2 kb双态条带的频率，在艾滋病来源的白色念珠菌和对照白色念珠菌之间没有显著差异。在疾病过程中的不同时间点以及从个体患者的不同部位分离出的白色念珠菌显示出相同的DNA指纹图谱。在艾滋病患者中引起疾病的白色念珠菌分离株与健康受试者中存在的白色念珠菌分离株之间的相似性表明，与艾滋病相关的念珠菌病不是由于存在独特的或特别有毒力的菌株，而是可能是宿主防御机制缺陷的结果。
