芽孢杆菌属土壤分离株对柴油、原油和废润滑油的生物降解作用
Biodegradation of Diesel, Crude Oil and Spent Lubricating Oil by Soil Isolates of Bacillus spp.
作者信息
Raju Maddela Naga, Leo Rodriguez, Herminia Sanaguano Salguero, Morán Ricardo Ernesto Burgos, Venkateswarlu Kadiyala, Laura Scalvenzi
机构信息
Department of Life Sciences, Universidad Estatal Amazónica, Puyo, Pastaza, 160150, Ecuador.
Faculty of Agriculture, Natural Resources and Environment, Universidad Estatal de Bolívar, Guaranda, Bolívar, 020150, Ecuador.
出版信息
Bull Environ Contam Toxicol. 2017 May;98(5):698-705. doi: 10.1007/s00128-017-2039-0. Epub 2017 Feb 16.
Two species of Bacillus, B. thuringiensis B3 and B. cereus B6, isolated from crude oil-contaminated sites in Ecuador, were tested for their capability in degrading polycyclic aromatic hydrocarbons (PAHs) in diesel (shake-flask), and to remove total petroleum hydrocarbons (TPHs) from crude oil- or spent lubricating oil-polluted soils (plot-scale). TPHs and PAHs were analyzed by Gas chromatography-Flame ionization detector (GC-FID) and High performance liquid chromatography (HPLC), respectively. Degradation percentages of PAHs by strain B6 were in the range of 11-83 after 30 days. A mixed culture of both the strains removed 84% and 28% of TPHs from crude oil- and spent lubricating oil-polluted soils, respectively. Reduction in the abundance of total n-alkane fractions (C-C) of spent lubricating oil was 94%, which was 18% higher than the control. Our results clearly indicate that the selected strains have great potential in degrading petroleum hydrocarbons at both laboratory- and field-scales.
从厄瓜多尔原油污染场地分离出的两种芽孢杆菌，即苏云金芽孢杆菌B3和蜡样芽孢杆菌B6，在柴油中降解多环芳烃（PAHs）的能力（摇瓶实验），以及从原油或废润滑油污染土壤中去除总石油烃（TPHs）的能力（小区规模实验），均进行了测试。TPHs和PAHs分别通过气相色谱 - 火焰离子化检测器（GC - FID）和高效液相色谱（HPLC）进行分析。30天后，菌株B6对PAHs的降解率在11% - 83%范围内。两种菌株的混合培养物分别从原油污染土壤和废润滑油污染土壤中去除了84%和28%的TPHs。废润滑油中总正构烷烃馏分（C - C）的丰度降低了94%，比对照高18%。我们的结果清楚地表明，所选菌株在实验室和现场规模降解石油烃方面具有巨大潜力。
Zotero 插件