Axel Meierjohann, Ewelina Kortesmäki, Jenny-Maria Brozinski, Leif Kronberg
Johan Gadolin Process Chemistry Centre, c/o Laboratory of Organic Chemistry, Åbo Akademi University, Piispankatu 8, FI-20500, Turku, Finland.
Environ Sci Pollut Res Int. 2017 Mar;24(9):8692-8699. doi: 10.1007/s11356-017-8588-2. Epub 2017 Feb 16.
A fast and simple method for the analysis of 17 commonly used antibiotics in Finland in water samples was developed. The method combines online solid phase extraction using a reusable online trapping column combined with analytical separation on a C analytical column and detection by a triple quadrupole mass spectrometer. The method was fully validated for detection and quantification limits as well as linearity, repeatability, and matrix effects. The method gave an excellent linear response (r > 0.99) and detection limits for all compounds (1-10 ng), except for tetracycline (20 ng l) and roxithromycin (50 ng l). The repeatability was evaluated at two concentrations, and the values at 5 ng l ranged from 5 to 39% and at 100 ng l ranged from 3 to 19%. To test the method on real samples at low environmental concentrations, water samples collected from a river receiving discharges from two wastewater treatment plants were analyzed as well as samples from a pristine river. Seven antibiotics as well as carbamazepine were detected in the samples. The concentration of the compounds ranged from 5 to 81 ng l.
开发了一种快速简便的方法，用于分析芬兰水样中17种常用抗生素。该方法将使用可重复使用的在线捕集柱进行在线固相萃取与在C分析柱上的分析分离以及通过三重四极杆质谱仪检测相结合。该方法在检测限、定量限以及线性、重复性和基质效应方面进行了全面验证。该方法对所有化合物（除四环素（20 ng/L）和罗红霉素（50 ng/L）外）给出了出色的线性响应（r > 0.99）和检测限（1 - 10 ng）。在两个浓度下评估了重复性，5 ng/L时的值范围为5%至39%，100 ng/L时的值范围为3%至19%。为了在低环境浓度下对实际样品测试该方法，分析了从接收两个废水处理厂排放的河流采集的水样以及来自原始河流的样品。在样品中检测到七种抗生素以及卡马西平。这些化合物的浓度范围为5至81 ng/L。
