Moraes Luis E, Blow Matthew J, Hawley Erik R, Piao Hailan, Kuo Rita, Chiniquy Jennifer, Shapiro Nicole, Woyke Tanja, Fadel James G, Hess Matthias
Department of Animal Science, University of California, Davis, 2251 Meyer Hall, Davis, CA, 95616, USA.
Department of Energy, Joint Genome Institute, Walnut Creek, CA, 94598, USA.
AMB Express. 2017 Dec;7(1):42. doi: 10.1186/s13568-017-0338-9. Epub 2017 Feb 16.
Cyanobacteria have the potential to produce bulk and fine chemicals and members belonging to Nostoc sp. have received particular attention due to their relatively fast growth rate and the relative ease with which they can be harvested. Nostoc punctiforme is an aerobic, motile, Gram-negative, filamentous cyanobacterium that has been studied intensively to enhance our understanding of microbial carbon and nitrogen fixation. The genome of the type strain N. punctiforme ATCC 29133 was sequenced in 2001 and the scientific community has used these genome data extensively since then. Advances in bioinformatics tools for sequence annotation and the importance of this organism prompted us to resequence and reanalyze its genome and to make both, the initial and improved annotation, available to the scientific community. The new draft genome has a total size of 9.1 Mbp and consists of 65 contiguous pieces of DNA with a GC content of 41.38% and 7664 protein-coding genes. Furthermore, the resequenced genome is slightly (5152 bp) larger and contains 987 more genes with functional prediction when compared to the previously published version. We deposited the annotation of both genomes in the Department of Energy's IMG database to facilitate easy genome exploration by the scientific community without the need of in-depth bioinformatics skills. We expect that an facilitated access and ability to search the N. punctiforme ATCC 29133 for genes of interest will significantly facilitate metabolic engineering and genome prospecting efforts and ultimately the synthesis of biofuels and natural products from this keystone organism and closely related cyanobacteria.
蓝藻有潜力生产大宗化学品和精细化学品，属于念珠藻属的成员因其相对较快的生长速度以及相对容易收获而受到特别关注。点状念珠藻是一种需氧、可运动、革兰氏阴性的丝状蓝藻，人们对其进行了深入研究，以增进我们对微生物碳和氮固定的理解。点状念珠藻模式菌株ATCC 29133的基因组于2001年进行了测序，自那时起科学界广泛使用了这些基因组数据。序列注释生物信息学工具的进展以及这种生物的重要性促使我们对其基因组进行重新测序和重新分析，并将初始注释和改进注释提供给科学界。新的基因组草图总大小为910万碱基对，由65个连续的DNA片段组成，GC含量为41.38%，有7664个蛋白质编码基因。此外，与之前发表的版本相比，重新测序的基因组略大（5152碱基对），包含功能预测的基因多了987个。我们将这两个基因组的注释存入了美国能源部的IMG数据库，以便科学界无需深入的生物信息学技能就能轻松进行基因组探索。我们预计，方便获取并能够在点状念珠藻ATCC 29133中搜索感兴趣的基因将显著促进代谢工程和基因组勘探工作，并最终促进从这种关键生物和密切相关的蓝藻中合成生物燃料和天然产物。
