Molecular Biology Laboratory - Post Graduate Program of Amazon Parasitic Biology, Biological and Health Sciences Center, State University of Pará, Belém, Pará, Brazil.
Molecular Biology Laboratory - Post Graduate Program of Amazon Parasitic Biology, Biological and Health Sciences Center, State University of Pará, Belém, Pará, Brazil.
Chemosphere. 2017 May;175:130-137. doi: 10.1016/j.chemosphere.2017.02.011. Epub 2017 Feb 3.
Aluminum and its compounds are common contaminants of water and food, as well as medications and cosmetics. The wide distribution of the element facilitates the demand for detailed studies of its biological and toxicological effects. This work aimed to evaluate the possible genotoxic and toxic activity resulting from in vivo and in vitro exposure to Al. For in vivo analysis, 40 Swiss mice were used, various concentrations of hydrated aluminum chloride were administered orally. They were analyzed for possible genic activity and metal cytotoxicity using a micronucleus test (MN), and for toxicity through histopathological evaluation of the extracted organs. For in vitro analysis, lymphocytes from the peripheral blood of 3 healthy donors were used. These cells were exposed to the same chemical agent in various concentrations. In vivo study revealed a significant increase in the number of MN in all Al concentrations. Furthermore, significant alterations in all the organs evaluated were verified by the presence of irreversible lesions (such as necrosis). Corroborating these findings, a significant increase in the quantity of MN in all concentrations with lymphocytes in vitro. In light of this, we suggest that this metal presents genotoxic potential and is potentially a cause of pathological disorders.
铝及其化合物是水和食物以及药物和化妆品的常见污染物。该元素的广泛分布促进了对其生物和毒理学效应的详细研究的需求。这项工作旨在评估由于体内和体外暴露于铝而产生的可能遗传毒性和毒性活性。在体内分析中，使用了 40 只瑞士小鼠，口服给予不同浓度的水合氯化铝。使用微核试验 (MN) 分析可能的基因活性和金属细胞毒性，并通过提取器官的组织病理学评估来分析毒性。在体外分析中，使用了来自 3 名健康供体的外周血淋巴细胞。这些细胞暴露于不同浓度的相同化学物质。体内研究表明，所有铝浓度的 MN 数量均显着增加。此外，通过存在不可逆损伤（如坏死）验证了所有评估器官的显着改变。体外淋巴细胞中所有浓度的 MN 数量的显着增加证实了这些发现。有鉴于此，我们建议该金属具有遗传毒性潜力，并且可能是病理紊乱的原因。
